. Instituto de Biotecnologia UNAM

IBT-UNAM : Dr. Carlos Felipe Peña Malacara

Dr. Carlos Felipe Peña Malacara

Investigador
Tutor de Maestría y Doctorado
Nivel II del SNI
Grupo del Dr. Enrique Galindo

email: carlosf @ibt.unam.mx
Teléfonos
Oficina +52 (777) 329-1777 ext 38305.
desde la CDMX 562-27777 ext 38305
red UNAM 38305

- Licenciatura: Biología, Fac. de Ciencias Biológicas UAEM (1986)
- Maestría: en Biotecnología, IBt-UNAM (1990)
- Doctorado: en Biotecnología, IBt-UNAM (1998)
- Reconocimiento como finalista del Premio Nacional en Tecnología de Alimentos, categoría profesional (1997)
- Estancia de Investigación: Universidad de Oviedo, España (1999-2000)
- Estancia de Investigación: Universidad Tecnológica de Aachen, Alemania (2003 y 2005)

Estudiantes

Q. Delia Areli Lima

Modesto Millan "Escalamiento- de fermentador de laboratorio de 1 L a fermentador piloto de 10 L- de la producción de alginato por Azotobacter vinelandii"

M.B Angie Johanna Torres

Sandra García

Tec. Alexis Jaciel Sánchez

Lic. Abraham Medina

IQ Jorge Alberto Sanchez

Ing. Manuel Alberto Castro "Establecimiento de un método de separación del POLI(3-HIDROXIBUTIRATO) producido por cepas mutantes de Azotobacter vinelandii."

Biol. Diana Tanairí Pérez

Publicaciones recientes

Castillo,T. Garcia,A. Padilla-Cordova,C. Diaz-Barrera,A. Pena,C. 2020. Respiration in Azotobacter vinelandii and its relationship with the synthesis of biopolymers Electronic Journal of Biotechnology, 48, 36-45.

Gomez-Hernandez,E. Salgado-Lugo,H. Segura,D. Garcia,A. Diaz-Barrera,A. Pena,C. 2020. Production of Poly-3-Hydroxybutyrate (P3HB) with Ultra-High Molecular Weight (UHMW) by Mutant Strains of Azotobacter vinelandii Under Microaerophilic Conditions Applied Biochemistry and Biotechnology, Aug 19 [Epub ahead of print], .

Velazquez-Sanchez,C. Espin,G. Pena,C. Segura,D. 2020. The Modification of Regulatory Circuits Involved in the Control of Polyhydroxyalkanoates Metabolism to Improve Their Production Frontiers in Bioengineering and Biotechnology, 8, 386.

Flores,C. Medina-Valdez,A. Pena,C. Serrano-Carreon,L. Galindo,E. 2020. Oxygen transfer rate determines molecular weight and production of poly(Î³-glutamic acid) as well as carbon utilization by Bacillus velezensis 83 Journal of Chemical Technology and Biotechnology, 95, 2383-2392.

Urtuvia,V. Maturana,N. Pena,C. Diaz-Barrera,A. 2020. Accumulation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Azotobacter vinelandii with different 3HV fraction in shake flasks and bioreactor Bioprocess and Biosystems Engineering, 43, 1469-1478.

Millan,C. Pena,C. Flores,C. Espin,G. Galindo,E. Castillo,T. 2020. Improving glucose and xylose assimilation in Azotobacter vinelandii by adaptive laboratory evolution World Journal Of Microbiology & Biotechnology, 36, 46.

Garcia,A. Castillo,T. Ramos,D. Ahumada-Manuel,C.L. Nunez,C. Galindo,E. Buchs,J. Pena,C. 2020. Molecular weight and viscosifying power of alginates produced by mutant strains of Azotobacter vinelandii under microaerophilic conditions Biotechnology Reports, 26, e00436.

Garcia,A. Perez,D. Castro,M. Urtuvia,V. Castillo,T. Diaz-Barrera,A. Espin,G. Pena,C. 2019. Production and recovery of poly-3-hydroxybutyrate (P(3HB)) of ultra-high molecular weight using fed-batch cultures of Azotobacter vinelandii OPNA strain Journal of Chemical Technology & Biotechnology, 94, 1853-1860.

Diaz-Barrera,A. Urtuvia,V. Padilla-Cordova,C. Pena,C. 2019. Poly(3-hydroxybutyrate) accumulation by Azotobacter vinelandii under different oxygen transfer strategies Journal of Industrial Microbiology & Biotechnology, 46, 13-19.

Castillo,T. Lopez,I. Flores,C. Segura,D. Garcia,A. Galindo,E. Pena,C. 2018. Oxygen uptake rate in alginate producer (algU+) and non-producer (algU-) strains of Azotobacter vinelandii under nitrogen-fixation conditions Journal of Applied Microbiology, 125, 181-189.

Adaya,L. Millan,M. Pena,C. Jendrossek,D. Espin,G. Tinoco-Valencia,R. Guzman,J. Pfeiffer,D. Segura,D. 2018. Inactivation of an intracellular poly-3-hydroxybutyrate depolymerase of Azotobacter vinelandii allows to obtain a polymer of uniform high molecular mass Applied Microbiology and Biotechnology, 102, 2693-2707.

Garcia,A. Ferrer,P. Albiol,J. Castillo,T. Segura,D. Pena,C. 2018. Metabolic flux analysis and the NAD(P)H/NAD(P)(+) ratios in chemostat cultures of Azotobacter vinelandii Microbial Cell Factories, 17, 10.

Barrales-Cureno,H.J. Sanchez-Herrera,L.M. Reyes-Reyes,C. Pena,C. Galindo,E. Gonzalez-Tena,P.A. de la Rosa-Tena,R. Lopez-Valdez,L.G. Cortes-Ruiz,J.A. 2017. Alginate production by Azotobacter vinelandi as a virtual sensor to estimate effective shear rate on stirred tank bioreactors Egyptian Journal of Experimental Biology (Bot.), 13, 321-331.

Urtuvia,V. Maturana,N. Acevedo,F. Pena,C. Diaz-Barrera,A. 2017. Bacterial alginate production: an overview of its biosynthesis and potential industrial production World Journal Of Microbiology & Biotechnology, 33, 198.

Millan,M. Salazar,M. Segura,D. Castillo,T. Diaz-Barrera,A. Pena,C. 2017. Molecular mass of Poly-3-hydroxybutyrate (P3HB) produced by Azotobacter vinelandii is influenced by the polymer content in the inoculum Journal of Biotechnology, 259, 50-55.

Castillo,T. Flores,C. Segura,D. Espin,G. Sanguino,J. Cabrera,E. Barreto,J. Diaz-Barrera,A. Pena,C. 2017. Production of polyhydroxybutyrate (PHB) of high and ultra-high molecular weight by Azotobacter vinelandii in batch and fed-batch cultures Journal of Chemical Technology & Biotechnology, 92, 1809-1816.

Ahumada-Manuel,C.L. Guzman,J. Pena,C. Quiroz-Rocha,E. Espin,G. Nunez,C. 2017. The signaling protein MucG negatively affects the production and the molecular mass of alginate in Azotobacter vinelandii Applied Microbiology and Biotechnology, 101, 1521-1534.

Abarca-Vargas,R. Pena Malacara,C.F. Petricevich,V.L. 2016. Characterization of Chemical Compounds with Antioxidant and Cytotoxic Activities in Bougainvillea x buttiana Holttum and Standl, (var. Rose) Extracts Antioxidants (Basel), 5, .

Millan,M. Segura,D. Galindo,E. Pena,C. 2016. Molecular mass of poly-3-hydroxybutyrate (P3HB) produced by Azotobacter vinelandii is determined by the ratio of synthesis and degradation under fixed dissolved oxygen tension Process Biochemistry, 51, 950-958.

Jimenez,L. Castillo,T. Flores,C. Segura,D. Galindo,E. Pena,C. 2016. Analysis of respiratory activity and carbon usage of a mutant of Azotobacter vinelandii impaired in poly-beta-hydroxybutyrate synthesis Journal of Industrial Microbiology & Biotechnology, 43, 1167-1174.

Diaz-Barrera,A. Andler,R. Martinez,I. Pena,C. 2016. Poly-3-hydroxybutyrate production by Azotobacter vinelandii strains in batch cultures at different oxygen transfer rates Journal of Chemical Technology and Biotechnology, 91, 1063-1071.

Gomez-Pazarin,K. Flores,C. Buchs,J. Castillo,T. Galindo,E. Pena,C. 2016. Molecular weight and viscosifying power of alginates produced in Azotobacter vinelandii cultures in shake flasks under low power input Journal of Chemical Technology and Biotechnology, 91, 1485-1492.

Flores,C. Diaz-Barrera,A. Martinez,F. Galindo,E. Pena,C. 2015. Role of oxygen in the polymerization and de-polymerization of alginate produced by Azotobacter vinelandii Journal of Chemical Technology & Biotechnology, 90, 356-365.

Dominguez-Diaz,M. Meneses-Acosta,A. Romo-Uribe,A. Pena,C. Segura,D. Espin,G. 2015. Thermo-mechanical properties, microstructure and biocompatibility in poly-beta-hydroxybutyrates (PHB) produced by OP and OPN strains of Azotobacter vinelandii European Polymer Journal, 63, 101-112.

Giese,H. Klockner,W. Pena,C. Galindo,E. Lotter,S. Wetzel,K. Meissner,L. Peter,C.P. Buchs,J. 2014. Effective shear rates in shake flasks Chemical Engineering Science, 118, 102-113.

Pena,C. Castillo,T. Garcia,A. Millan,M. Segura,D. 2014. Biotechnological strategies to improve production of microbial poly-(3-hydroxybutyrate): a review of recent research work Microbial Biotechnology, 7, 278-293.

Garcia,A. Segura,D. Espin,G. Galindo,E. Castillo,T. Pena,C. 2014. High production of poly-beta-hydroxybutyrate (PHB) by an Azotobacter vinelandii mutant altered in PHB regulation using a fed-batch fermentation process Biochemical Engineering Journal, 82, 117-123.

Pena,C. Lopez,S. Garcia,A. Espin,G. Romo-Uribe,A. Segura,D. 2014. Biosynthesis of poly-b-hydroxybutyrate (PHB) with a high molecular mass by a mutant strain of Azotobacter vinelandii (OPN) Annals of Microbiology, 64, 39-47.

Flores,C. Moreno,S. Espin,G. Pena,C. Galindo,E. 2013. Expression of alginases and alginate polymerase genes in response to oxygen, and their relationship with the alginate molecular weight in Azotobacter vinelandii Enzyme and Microbial Technology, 53, 85-91.

Castillo,T. Heinzle,E. Peifer,S. Schneider,K. Pena M,C.F. 2013. Oxygen supply strongly influences metabolic fluxes, the production of poly(3-hydroxybutyrate) and alginate, and the degree of acetylation of alginate in Azotobacter vinelandii Process Biochemistry, 48, 995-1003.

Castillo,T. Galindo,E. Pena,C.F. 2013. The acetylation degree of alginates in Azotobacter vinelandii ATCC9046 is determined by dissolved oxygen and specific growth rate: studies in glucose-limited chemostat cultivations Journal of Industrial Microbiology & Biotechnology, 40, 715-723.

Nunez,C. Pena,C. Kloeckner,W. Hernandez-Eligio,A. Bogachev,A.V. Moreno,S. Guzman,J. Buchs,J. Espin,G. 2013. Alginate synthesis in Azotobacter vinelandii is increased by reducing the intracellular production of ubiquinone Applied Microbiology and Biotechnology, 97, 2503-2512.

Gaytan,I. Pena,C. Nunez,C. Cordova,M.S. Espin,G. Galindo,E. 2012. Azotobacter vinelandii lacking the Na +-NQR activity: a potential source for producing alginates with improved properties and at high yield World Journal Of Microbiology & Biotechnology, 28, 2731-2740.

Lozano,E. Galindo,E. Pena,C.F. 2011. Oxygen transfer rate during the production of alginate by Azotobacter vinelandii under oxygen-limited and non oxygen-limited conditions Microbial Cell Factories, 10, 13.

Pena,C. Galindo,E. Buchs,J. 2011. The viscosifying power, degree of acetylation and molecular mass of the alginate produced by Azotobacter vinelandii in shake flasks are determined by the oxygen transfer rate Process Biochemistry, 46, 290-297.

Mejia,M.A. Segura,D. Espin,G. Galindo,E. Pena,C. 2010. Two-stage fermentation process for alginate production by Azotobacter vinelandii mutant altered in poly-beta-hydroxybutyrate (PHB) synthesis Journal of Applied Microbiology, 108, 55-61.

Coronado,E. Galindo,E. Pena,C. 2008. Papel del alginato en la agregación de Azotobacter vinelandii en cultivo sumergido Revista Colombiana de Biotecnología, 10, 10-16.

Pena,C. Millan,M. Galindo,E. 2008. Production of alginate by Azotobacter vinelandii in a stirred fermentor simulating the evolution of power input observed in shake flasks Process Biochemistry, 43, 775-778.

Pena,C. Peter,C.P. Buchs,J. Galindo,E. 2007. Evolution of the specific power consumption and oxygen transfer rate in alginate-producing cultures of Azotobacter vinelandii conducted in shake flasks Biochemical Engineering Journal, 36, 73-80.

Diaz-Barrera,A. Pena,C. Galindo,E. 2007. The oxygen transfer rate influences the molecular mass of the alginate produced by Azotobacter vinelandii Applied Microbiology and Biotechnology, 76, 903-910.

Galindo,E. Pena,C. Nunez,C. Segura,D. Espin,G. 2007. Molecular and bioengineering strategies to improve alginate and polydydroxyalkanoate production by Azotobacter vinelandii Microbial Cell Factories, 6, 7.

Pena,C. Hernandez,L. Galindo,E. 2006. Manipulation of the acetylation degree of Azotobacter vinelandii alginate by supplementing the culture medium with 3-(N-morpholino)-propane-sulfonic acid Letters in Applied Microbiology, 43, 200-204.

Priego-Jimenez,R. Pena,C. Ramirez,O.T. Galindo,E. 2005. Specific growth rate determines the molecular mass of the alginate produced by Azotobacter vinelandii Biochemical Engineering Journal, 25, 187-193.

Reyes,C. Pena,C. Galindo,E. 2003. Reproducing shake flasks performance in stirred fermentors: production of alginates by Azotobacter vinelandii Journal of Biotechnology, 105, 189-198.

Trujillo-Roldan,M.A. Pena,C. Galindo,E. 2003. Components in the inoculum determine the kinetics of Azotobacter vinelandii cultures and the molecular weight of its alginate Biotechnology Letters, 25, 1251-1254.

Petricevich,V.L. Pena,C.F. 2002. The dynamics of cytokine d nitric oxide secretion in mice injected with Tityus serrulatus scorpion venom Mediators of inflammation, 11, 173-180.

Pena,C. Miranda,L. Segura,D. Nunez,C. Espin,G. Galindo,E. 2002. Alginate production by Azotobacter vinelandii mutants altered in poly-beta-hydroxybutyrate and alginate biosynthesis Journal of Industrial Microbiology & Biotechnology, 29, 209-213.

Pena,C. Reyes,C. Larralde-Corona,P. Corkidi,G. Galindo,E. 2002. Characterization of Azotobacter vinelandii aggregation in submerged culture by digital image analysis Fems Microbiology Letters, 207, 173-177.

Pena,C. Galindo,E. Diaz,M. 2002. Effectiveness factor in biological external convection: study in high viscosity systems Journal of Biotechnology, 95, 1-12.

Trujillo-Roldan,M.A. Pena,C. Ramirez,O.T. Galindo,E. 2001. Effect of Oscillating Dissolved Oxygen Tension on the Production of Alginate by Azotobacter vinelandii Biotechnology Progress, 17, 1042-1048.

Seanez,G. Pena,C. Galindo,E. 2001. High CO2 affects alginate production and prevents polymer degradation in cultures of Azotobacter vinelandii Enzyme and Microbial Technology, 29, 535-540.

Pena,C. Trujillo-Roldan,M.A. Galindo,E. 2000. Influence of dissolved oxygen tension and agitation speed on alginate production and its molecular weight in cultures of Azotobacter vinelandii Enzyme and Microbial Technology, 27, 390-398.

Pena,C. Campos,N. Galindo,E. 1997. Changes in alginate molecular mass distributions, broth viscosity and morphology of Azotobacter vinelandii cultured in shake flasks Applied Microbiology and Biotechnology, 48, 510-515.

Casas,L. Pena,C. Salvador,M. Frias,P. 1992. Influence of spray drying on the permeability of K. fragilis measured by beta-galactosidase activity Food Biotechnology, 6, 135-152.

Gonzalez,M. Pena,C. Casas,L.T. 1990. PARTIAL-PURIFICATION OF BETA-GALACTOSIDASE FROM YEAST BY AN AQUEOUS 2-PHASE SYSTEM METHOD.Process Biochemistry, 25, 157-161.

Libros y capítulos recientes

Pena-Malacara,C. Garcia-Romero,A. Millan-Ponce,M. Castillo-Marenco,T. 2016. Approaches for the synthesis of tailor-made polyhydroxyalkanoates (PHAs) en: Kalia,V.C. Microbial Factories: Biodiversity, Biofuels, Biopolymers, Bioactive Molecules and Waste Treatment. Springer India. pags. 11-28

Castillo-Marenco,T. Pena-Malacara,C. 2014. Geles, espesantes y una bacteria fascinante en: Rebolledo,F. Un Mundo de Bacterias. UNAM, Campus Morelos. pags. 87-90

Romero,Y. Pena,C. Rojas,A. Tortajada,M. Espin,G. Segura,D. 2014. Una mutante de Azotobacter vinelandii productora de PHB que carece de los en: Mendez,B. Bioplasticos. Lisboa, Portugal. Editorial CYTED. pags. 23-31

Pena,C. Castillo,T. Segura,D. Nunez,C. 2011. Bioprocess design: fermentation strategies for improving the production of alginate and poly-beta-hydroxybutyrate (PHB) by Azotobacter vinelandii en: Carpi,A. Progress in Molecular and Environmental Bioengineering. Intech. pags. 217-242

Galindo,E. Pena,C. Serrano-Carreon,L. 2008. Domesticar microorganismos en un biorreactor: los retos del bioingeniero en: *agustin Una ventana al quehacer científico. Instituto de Biotecnología de la UNAM 25 aniversario, cap 12. Mexico, D.F.. UNAM. pags. 131-143

Otras Publicaciones

Olalde-Quintanar,I. Pena-Malacara,C. 2018. Innovación con Ciencia: Una iniciativa que busca promover Biotecnología en Movimiento.Revista de divulgación del Instituto de Biotecnología de la UNAM, 12, 20-24.

Millan-Ponce,M. Pena-Malacara,C.F. 2017. Producir plásticos biodegradables: un reto de la biotecnología Biotecnología en Movimiento.Revista de divulgación del Instituto de Biotecnología de la UNAM, 8, 3-5.

Pena-Malacara,C.F. 2015. Innovación y emprendimiento de base tecnológica en el Campus Morelos de la UNAM Biotecnología en Movimiento.Revista de divulgación del Instituto de Biotecnología de la UNAM, 3, 18-19.

Pena-Malacara,C. Fuentes-Ponce,G. 2015. Entrenando a estudiantes en emprendimiento de base científica [Este artículo fue publicado originalmente en el periódico "La Unión de Morelos", 18 de febrero de 2015, pag. 12.] Biotecnología en Movimiento.Revista de divulgación del Instituto de Biotecnología de la UNAM, 1, 24-25.

Pena-Malacara,C. Garcia-Romero,A. 2012. La belleza de una bacteria, el caso de Azotobacter vinelandii. Hypatia, 42, .

Pena-Malacara,C. Segura-Gonzalez,D. 2011. Geles y plásticos hechos bajo diseño por fermentación bacteriana Hypatia, 35, 4-5.

Trujillo-Roldan,M.A. Pena,C. Galindo,E. 2005. A model predicting the effects of oscillating dissolved oxygen tension on the molecular weight of alginate by azotobacter vinelandii Revista Mexicana de Ingenieria Quimica, 4, 37-45.

Pena,C. Galindo,E. 2002. Las condiciones de cultivo determinan las caracteristicas moleculares de los polisacaridos microbianso Biotecnologia, 7, 22-28.