. Instituto de Biotecnologia UNAM

IBT-UNAM : Dra. Patricia Ileana Joseph Bravo

Dra. Patricia Ileana Joseph Bravo

Líder Académico »» Grupo
Investigador
Tutor de Maestría y Doctorado
Nivel III del SNI
Departamento de Genética del Desarrollo y Fisiología Molecular

Líneas de investigación

email: joseph @ibt.unam.mx
Teléfonos
Oficina +52 (777) 329-1632
desde la CDMX 562-27632
red UNAM 27632
Fax +52 (777) 3170805

Laboratorio +52 (777) 329-1620
desde la CDMX 562-27620
red UNAM 27620

- Licenciatura: Química, Fac. de Química-UNAM (1970)
- Maestría: en Nutricion y Metabolismo. Mass. Inst. of Technology, Cambridge, Mass E.U.A. Dept. de Ciencia en Nutrición y Metabolismo (1974)
- Doctorado: en Bioquímica, Universidad de Londres, Colegio Imperial de Ciencia y Tecnología , Depto. de Bioquímica, Londres, Inglaterra (1978)

Miembro del Consejo Consultivo de Posgrado de la UNAM (2003)
Distinción Juana Ramírez de Abaje UNAM (2003)
Premio Miguel Aleman (1988)

Estudiantes

Lic. Angelica Gutierrez

Biol. Karen Lissette Garduño

M.C. Andrea Castillo

M.C. Marco Antonio Parra

M.C. Adrian Perez

Publicaciones recientes

Castillo-Campos,A. Gutierrez-Mata,A. Charli,J.L. Joseph-Bravo,P. 2020. Chronic stress inhibits hypothalamus-pituitary-thyroid axis and brown adipose tissue responses to acute cold exposure in male rats Journal of Endocrinological Investigation, 30 July online first, .

Charli,J.L. Rodriguez-Rodriguez,A. Hernandez-Ortega,K. Cote-Velez,A. Uribe,R.M. Jaimes-Hoy,L. Joseph-Bravo,P. 2020. The Thyrotropin-Releasing Hormone-Degrading Ectoenzyme, a Therapeutic Target? Frontiers in Pharmacology, 11, 640.

Joseph-Bravo,P. Lazcano,I. Jaimes-Hoy,L. Gutierrez-Mariscal,M. Sanchez-Jaramillo,E. Uribe,R.M. Charli,J.L. 2020. Sexually dimorphic dynamics of thyroid axis activity during fasting in rats Frontiers in Bioscience, Landmark, 25, 1305-1323.

Farkas,E. Varga,E. Kovacs,B. Szilvasy-Szabo,A. Cote-Velez,A. Peterfi,Z. Matziari,M. Toth,M. Zelena,D. Mezriczky,Z. Kadar,A. Kovari,D. Watanabe,M. Kano,M. Mackie,K. Rozsa,B. Ruska,Y. Toth,B. Mate,Z. Erdelyi,F. Szabo,G. Gereben,B. Lechan,R.M. Charli,J.L. Joseph-Bravo,P. Fekete,C. 2020. A glial-neuronal circuit in the median eminence regulates thyrotropin-releasing hormone-release via the endocannabinoid system iScience, 23, 100921.

Jaimes-Hoy,L. Romero,F. Charli,J.L. Joseph-Bravo,P. 2019. Sex Dimorphic Responses of the Hypothalamus-Pituitary-Thyroid Axis to Maternal Separation and Palatable Diet Frontiers in Endocrinology, 10, 445.

Rodriguez-Rodriguez,A. Lazcano,I. Sanchez-Jaramillo,E. Uribe,R.M. Jaimes-Hoy,L. Joseph-Bravo,P. Charli,J.L. 2019. Tanycytes and the Control of Thyrotropin-Releasing Hormone Flux Into Portal Capillaries Frontiers in Endocrinology, 10, 401.

Parra-Montes de Oca,M.A. Gutierrez-Mariscal,M. Salmeron-Jimenez,M.F. Jaimes-Hoy,L. Charli,J.L. Joseph-Bravo,P. 2019. Voluntary Exercise-Induced Activation of Thyroid Axis and Reduction of White Fat Depots Is Attenuated by Chronic Stress in a Sex Dimorphic Pattern in Adult Rats Frontiers in Endocrinology, 10, 418.

Jaimes-Hoy,L. Gurrola,G.B. Cisneros,M. Joseph-Bravo,P. Possani,L.D. Charli,J.L. 2017. The Kv1.3 channel blocker Vm24 enhances muscle glucose transporter 4 mobilization but does not reduce body-weight gain in diet-induced obese male rats Life Sciences, 181, 23-30.

Gonzalez-Franco,D.A. Ramirez-Amaya,V. Joseph-Bravo,P. Prado-Alcala,R.A. Quirarte,G.L. 2017. Differential Arc protein expression in dorsal and ventral striatum after moderate and intense inhibitory avoidance training Neurobiology of Learning and Memory, 140, 17-26.

Cote-Velez,A. Martinez-Baez A. Lezama,L. Uribe,R.M. Joseph-Bravo,P. Charli,J.L. 2017. A screen for modulators reveals that orexin-A rapidly stimulates thyrotropin releasing hormone expression and release in hypothalamic cell culture Neuropeptides, 62, 11-20.

Sotelo-Rivera,I. Cote-Velez,A. Uribe,R.M. Charli,J.L. Joseph-Bravo,P. 2017. Glucocorticoids curtail stimuli-induced CREB phosphorylation in TRH neurons through interaction of the glucocorticoid receptor with the catalytic subunit of protein kinase A Endocrine, 55, 861-871.

Joseph-Bravo,P. Jaimes-Hoy,L. Charli,J.L. 2016. Advances in TRH signaling Reviews in Endocrine and Metabolic Disorders, 17, 545-558.

Jaimes-Hoy,L. Gutierrez-Mariscal,M. Vargas,Y. Perez-Maldonado,A. Romero,F. Sanchez-Jaramillo,E. Charli,J.L. Joseph-Bravo,P. 2016. Neonatal maternal separation alters, in a sex specific manner, the expression of TRH, of TRH-degrading ectoenzyme in the rat hypothalamus, and the response of the thyroid axis to starvation Endocrinology, 157, 3253-3265.

Joseph-Bravo,P. Jaimes-Hoy,L. Uribe,R.M. Charli,J.L. 2015. TRH, first hypophysiotropic releasing hormone isolated: its control of pituitary-thyroid axis Journal of Endocrinology, 226, T85-T100 [erratum en vol 227 (3) X3].

Lazcano,I. Cabral,A. Uribe,R.M. Jaimes-Hoy,L. Perello,M. Joseph-Bravo,P. Sanchez-Jaramillo,E. Charli,J.L. 2015. Fasting enhances pyroglutamyl peptidase II activity in tanycytes of the mediobasal hypothalamus of male adult rats Endocrinology, 156, 2713-2723.

Joseph-Bravo,P. Jaimes-Hoy,L. Charli,J. 2015. Regulation of TRH neurons and energy homeostasis related signals under stress Journal of Endocrinology, 224, R139-R159 [erratum en vol 227 (3) X1].

Sotelo-Rivera,I. Jaimes-Hoy,L. Cote-Velez,A. Espinoza-Ayala,C. Charli,J.L. Joseph-Bravo,P. 2014. An acute injection of corticosterone increases thyrotrophin releasing hormone expression in the paraventricular nucleus of the hypothalamus but interferes with the rapid hypothalamus pituitary thyroid axis response to cold in male rats Journal of Neuroendocrinology, 26, 861-869.

Rodriguez-Molina,V. Patino,J. Vargas,Y. Sanchez-Jaramillo,E. Joseph-Bravo,P. Charli,J.L. 2014. TRH regulates action potential shape in cerebral cortex pyramidal neurons Brain Research, 1571, 1-11.

Uribe,R.M. Jaimes-Hoy,L. Ramirez-Martinez,C. Garcia-Vazquez,A. Romero,F. Cisneros,M. Cote-Velez,A. Charli,J.L. Joseph-Bravo,P. 2014. Voluntary exercise adapts the hypothalamus-pituitary-thyroid axis in male rats Endocrinology, 155, en20131724.

Gutierrez-Mariscal,M. Sanchez,E. Garcia-Vazquez,A. Rebolledo-Solleiro,D. Charli,J.L. Joseph-Bravo,P. 2012. Acute response of hypophysiotropic thyrotropin releasing hormone neurons and thyrotropin Regulatory Peptides, 179, 61-70.

Lazcano,I. Uribe,R.M. Martinez-Chavez,E. Vargas,M.A. Matziari,M. Joseph-Bravo,P. Charli,J.L. 2012. Pyroglutamyl peptidase II inhibition enhances the analeptic effect of thyrotropin releasing hormone in the rat medial septum Journal of Pharmacology and Experimental Therapeutics, 342, 222-231.

Gutierrez-Mariscal,M. Sanchez,E. Rebolledo-Solleiro,D. Garcia-Vazquez,A.I. Cote-Velez,A. Acasuso-Rivero,C. Charli,J.L. Joseph-Bravo,P. 2012. The acute response of the amygdalar TRH system to psychogenic stressors varies dependent on the paradigm and circadian condition Brain Research, 1452, 73-84.

Guerra-Crespo,M. Perez-Monter,C. Janga,S.C. Castillo-Ramirez,S. Gutierrez-Rios,R.M. Joseph-Bravo,P. Perez-Martinez,L. Charli,J.L. 2011. Transcriptional profiling of fetal hypothalamic TRH neurons BMC Genomics, 12, 222.

Cote-Velez,A. Perez-Maldonado,A. Osuna,J. Barrera,B. Charli,J.L. Joseph-Bravo,P. 2011. Creb and Sp/Kruppel response elements cooperate to control rat TRH gene transcription in response to cAMP Biochimica et Biophysica Acta (BBA), 1809, 191-199.

Uribe,R.M. Cisneros,M. Vargas,M.A. Lezama,L. Cote-Velez,A. Joseph-Bravo,P. Charli,J.L. 2011. The systemic inhibition of nitric oxide production rapidly regulates TRH mRNA concentration in the paraventricular nucleus of the hypothalamus and serum TSH concentration. Studies in control and cold-stressed rats Brain Research, 1367, 188-197.

Diaz-Gallardo,M.Y. Cote-Velez,A. Charli,J.L. Joseph-Bravo,P. 2010. A rapid interference between glucocorticoids and cAMP-activated signaling in hypothalamic neurons prevents binding of pCREB and GR at the CRE-like and composite GRE sites of TRH gene promoter Journal of Neuroendocrinology, 22, 282-293.

Diaz-Gallardo,M.Y. Cote-Velez,A. Carreon-Rodriguez,A. Charli,J.L. Joseph-Bravo,P. 2010. Phosphorylated Cyclic-AMP-Response Element-Binding Protein and Thyroid Hormone Receptor Have Independent Response Elements in the Rat Thyrotropin-Releasing Hormone Promoter: An Analysis in Hypothalamic Cells Neuroendocrinology, 91, 64-76.

Uribe,R.M. Zacarias,M. Corkidi,G. Cisneros,M. Charli,J.L. Joseph-Bravo,P. 2009. 17beta-oestradiol indirectly inhibits trh expression in the hypothalamic paraventricular nucleus of female rats and blunts thyroid axis response to cold exposure Journal of Neuroendocrinology, 21, 439-448.

Rodriguez-Molina,V. Vargas,M.A. Joseph-Bravo,P. Charli,J.L. 2009. NMDA receptor up-regulates pyroglutamyl peptidase II activity in the rat hippocampus Neuroscience Letters, 449, 211-214.

de Gortari,P. Mancera,K. Cote-Velez,A. Amaya,M.I. Martinez,A. Jaimes-Hoy,L. Joseph-Bravo,P. 2009. Involvement of CRH-R2 receptor in eating behavior and in the response of the HPT axis in rats subjected to dehydration-induced anorexia Psychoneuroendocrinology, 34, 259-272.

Cruz,R. Vargas,M.A. Uribe,R.M. Pascual,I. Lazcano,I. Yiotakis,A. Matziari,M. Joseph-Bravo,P. Charli,J.L. 2008. Anterior pituitary pyroglutamyl peptidase II activity controls TRH-induced prolactin release Peptides, 29, 1953-1964.

Cote-Velez,A. Perez-Martinez,L. Charli,J.L. Joseph-Bravo,P. 2008. The PKC and ERK/MAPK Pathways Regulate Glucocorticoid Action on TRH Transcription Neurochemical Research, 33, 1582-1591.

Jaimes-Hoy,L. Joseph-Bravo,P. de Gortari,P. 2008. Differential response of TRHergic neurons of the hypothalamic paraventricular nucleus (PVN) in female animals submitted to food-restriction or dehydration-induced anorexia and cold exposure Hormones and Behavior, 53, 366-377.

Gutierrez-Mariscal,M. de Gortari,P. Lopez-Rubalcava,C. Martinez,A. Joseph-Bravo,P. 2008. Analysis of the anxiolytic-like effect of TRH and the response of amygdalar TRHergic neurons in anxiety Psychoneuroendocrinology, 33, 198-213.

de Gortari,P. Vargas,M.A. Martinez,A. Garcia-Vazquez,A.I. Uribe,R.M. Chavez-Gutierrez,L. Magdaleno,V. Boileau,G. Charli,J.L. Joseph-Bravo,P. 2007. Stage-specific Modulation of Neprilysin and Aminopeptidase N in the Limbic System During Kindling Progression Journal of Molecular Neuroscience, 33, 252-261.

Ubieta,R. Uribe,R.M. Gonzalez,J.A. Garcia-Vazquez,A. Perez-Monter,C. Perez-Martinez,L. Joseph-Bravo,P. Charli,J.L. 2007. BDNF up-regulates pre-pro-TRH mRNA expression in the fetal/neonatal paraventricular nucleus of the hypothalamus. Properties of the transduction pathway Brain Research, 1174, 28-38.

Sanchez,E. Fekete,C. Lechan,R.M. Joseph-Bravo,P. 2007. Cocaine- and amphetamine-regulated transcript (CART) expression is differentially regulated in the hypothalamic paraventricular nucleus of lactating rats exposed to suckling or cold stimulation Brain Research, 1132, 120-128.

Aguilar-Valles,A. Sanchez,E. de Gortari,P. Garcia-Vazquez,A.I. Ramirez-Amaya,V. Bermudez-Rattoni,F. Joseph-Bravo,P. 2007. The expression of TRH, its receptors and degrading enzyme is differentially modulated in the rat limbic system during training in the Morris water maze Neurochemistry International, 50, 404-417.

Chavez-Gutierrez,L. Matta-Camacho,E. Osuna,J. Horjales,E. Joseph-Bravo,P. Maigret,B. Charli,J.L. 2006. Homology modeling and site directed mutagenesis of pyroglutamyl peptidase II. Insights into omega versus aminopeptidase specificity in the M1 family Journal of Biological Chemistry, 281, 18581-18590.

de Gortari,P. Uribe,R.M. Garcia-Vazquez,A. Aguilar-Valles,A. Martinez,A. Valdes,A. Charli,J.L. Fernandez-Guardiola,A. Joseph-Bravo,P. 2006. Amygdala kindling differentially regulates the expression of the elements involved in TRH transmission Neurochemistry International, 48, 31-42.

Aguilar-Valles,A. Sanchez,E. de Gortari,P. Balderas,I. Ramirez-Amaya,V. Bermudez-Rattoni,F. Joseph-Bravo,P. 2005. Analysis of the Stress Response in Rats Trained in the Water-Maze: Differential Expression of Corticotropin-Releasing Hormone, CRH-R1, Glucocorticoid Receptors and Brain-Derived Neurotrophic Factor in Limbic Regions Neuroendocrinology, 82, 306-319.

de Gortari,P. Romero,F. Cisneros,M. Joseph-Bravo,P. 2005. Acute administration of alcohol modulates pyroglutamyl amino peptidase II activity and mRNA levels in rat limbic regions Neurochemistry International, 46, 347-356.

Cote-Velez,A. Perez-Martinez,L. Diaz-Gallardo,M.Y. Perez-Monter,C. Carreon,A. Charli,J.L. Joseph-Bravo,P. 2005. Dexamethasone repressed cAMP rapid upregulation of TRH gene transcription. Identification of a composite GRE and a CRE in TRH promoter Journal of Molecular Endocrinology, 34, 177-197.

de Gortari,P. Cisneros,M. Joseph-Bravo,P. 2005. Chronic ethanol or glucose consumption alter TRH content and pyroglutamyl aminopeptidase II activity in rat limbic regions Regulatory Peptides, 127, 141-150.

Chavez-Gutierrez,L. Bourdais,J. Aranda,G. Vargas,M.A. Matta-Camacho,E. Ducancel,F. Segovia,L. Joseph-Bravo,P. Charli,J.L. 2005. A truncated isoform of pyroglutamyl aminopeptidase II produced by exon extension has dominant-negative activity Journal of Neurochemistry, 92, 807-817.

Pascual,I. Cisneros,M. Lage,J. Diaz,J. Joseph-Bravo,P. Charli,J.L. Chavez,M.A. 2004. Isolation and purification of HcPI, a natural inhibitor of pyroglutamil aminopeptidase II (TRH-degrading ectoenzyme) from extracts of Hermodice carunculata (annelide: Polychaeta) Revista Biologia, 18, 38-49.

Cruz,R. Chavez-Gutierrez,L. Joseph-Bravo,P. Charli,J.L. 2004. 3,3',5'-Triiodo-L-Thyronine Reduces Efficiency of mRNA Knockdown by Antisense Oligodeoxynucleotides: A Study with Pyroglutamyl Aminopeptidase II in Adenohypophysis Oligonucleotides, 14, 176-190.

Joseph-Bravo,P. 2004. Hypophysiotropic thyrotropin-releasing hormone neurons as transducers of energy homeostasis Endocrinology, 145, 4813-4815.

Pascual,I. Gil-Parrado,S. Cisneros,M. Joseph-Bravo,P. Diaz,J. Possani,L.D. Charli,J.L. Chavez,M. 2004. Purification of a specific inhibitor of pyroglutamyl aminopeptidase II from the marine annelide Hermodice carunculata. In vivo effects in rodent brain International Journal of Biochemistry and Cell Biology, 36, 138-152.

Vargas,M.A. Uribe,R.M. Cisneros,M. Romero,F. Gonzalez,S. Joseph-Bravo,P. Charli,J.L. 2002. Thyrotropin-releasing hormone regulates the diurnal variation of pyroglutamyl aminopeptidase II activity in the male rat adenohypophysis European Journal of Endocrinology, 147, 363-369.

Vargas,M.A. Cisneros,M. Joseph-Bravo,P. Charli,J.L. 2002. Thyrotropin-releasing hormone-induced down-regulation of pyroglutamyl aminopeptidase II activity involves L-type calcium channels and cam kinase activities in cultures of adenohypophyseal cells Journal of Neuroendocrinology, 14, 184-193.

de Gortari,P. Cisneros,M. Medellin,M.A. Joseph-Bravo,P. 2002. Chronic ingestion of ethanol or glucose solutions affects hypothalamic and limbic TRH metabolism in dams and their pups Neurochemistry International, 41, 237-249.

Joseph-Bravo,P. Perez-Martinez,L. Lezama,L. Morales-Chapa,C. Charli,J.L. 2002. An improved method for the expression of TRH in serum-supplemented primary cultures of fetal hypothalamic cells Brain Research Protocols, 9, 93-104.

Sanchez,E. Uribe,R.M. Corkidi,G. Zoeller,R.T. Cisneros,M. Zacarias,M. Morales-Chapa,C. Charli,J.L. Joseph-Bravo,P. 2001. Differential Responses of Thyrotropin-Releasing Hormone (TRH) Neurons to Cold Exposure or Suckling Indicate Functional Heterogeneity of the TRH System in the Paraventricular Nucleus of the Rat Hypothalamus Neuroendocrinology, 74, 407-422.

Guerra-Crespo,M. Ubieta,R. Joseph-Bravo,P. Charli,J.L. Perez-Martinez,L. 2001. BDNF increases the early expression of TRH mRNA in fetal TrkB+ hypothalamic neurons in primary culture European Journal of Neuroscience, 14, 483-494.

Perez-Martinez,L. Charli,J. Joseph-Bravo,P. 2001. Development of pro-TRH gene expression in primary cultures of fetal hypothalamic cells Developmental Brain Research, 130, 73-81.

Baeza,M.A. Ponce,G. Joseph-Bravo,P. Charli,J.L. 2001. Rapid down regulation of pyroglutamyl peptidase II activity by arachidonic acid in primary cultures of adenohypophyseal cells Life Sciences, 68, 2051-2060.

Niquet,J. Perez-Martinez,L. Guerra,M. Grouselle,D. Joseph-Bravo,P. Charli,J. 2000. Extracellular matrix proteins increase the expression of pro-TRH and pro-protein convertase PC1 in fetal hypothalamic neurons in vitro Developmental Brain Research, 120, 49-56.

de Gortari,P. Mendez,M. Rodriguez-Keller,I. Perez-Martinez,L. Joseph-Bravo,P. 2000. Acute ethanol administration induces changes in TRH and proenkephalin expression in hypothalamic and limbic regions of rat brain Neurochemistry International, 37, 483-496.

Bourdais,J. Romero,F. Uriostegui,B. Cisneros,M. Joseph-Bravo,P. Charli,J.L. 2000. [3-Me-His(2)]-TRH combined with dopamine withdrawal rapidly and transiently increases pyroglutamyl aminopeptidase II activity in primary cultures of adenohypophyseal cells Neuropeptides, 34, 83-88.

Vargas,M.A. Cisneros,M. Joseph-Bravo,P. Charli,J.L. 2000. Regulation of adenohypophyseal pyroglutamyl aminopeptidase II activity by thyrotropin-releasing hormone and phorbol esters Endocrine, 13, 267-272.

de Gortari,P. Gonzalez-Alzati,M.E. Cisneros,M. Joseph-Bravo,P. 2000. Effect of fasting on the content of thyrotropin-releasing hormone and its mRNA in the central nervous system and pyroglutamyl peptidase II activity in the anterior pituitary of post-weaned and adult rats.Nutritional Neuroscience, 3, 255-265.

Perez-Martinez,L. Uribe,R.M. Sanchez,E. Carreon-Rodriguez,A. Chavez,L. Zacarias,M. Joseph-Bravo,P. Charli,J.L. 1999. Regulation of TRH biosynthesis in the paraventricular nucleus of rodent hypothalamus Current topics in neurochemistry, 2, 79-88.

Mendez,M. Cisneros,M. Baez,A. Joseph-Bravo,P. Charli,J.L. 1999. Three TRH-like molecules are released from rat hypothalamus in vitro Neurochemical Research, 24, 815-823.

de Gortari,P. Joseph-Bravo,P. Monroy-Ruiz,J. Martinez,A. Cisneros,M. Fernandez-Guardiola,A. 1998. Brain thyrotropin-releasing hormone content varies through amygdaloid kindling development according to afterdischarge frequency and propagation Epilepsia, 39, 897-903.

Joseph-Bravo,P. Uribe,R.M. Vargas,M.A. Perez-Martinez,L. Zoeller,T. Charli,J.L. 1998. Multifactorial modulation of TRH metabolism Cellular & Molecular Neurobiology, 18, 231-247.

Vargas,M.A. Bourdais,J. Sanchez,S. Uriostegui,B. Moreno,E. Joseph-Bravo,P. Charli,J.L. 1998. Multiple hypothalamic factors regulate pyroglutamyl peptidase II in cultures of adenohypophyseal cells: role of the cAMP pathway Journal of Neuroendocrinology, 10, 199-206.

Charli,J.L. Vargas,M.A. Cisneros,M. de Gortari,P. Baeza,M.A. Jasso,P. Bourdais,J. Perez,L. Uribe,R.M. Joseph-Bravo,P. 1998. TRH inactivation in the extracellular compartment: role of pyroglutamyl peptidase II.Neurobiology (Bp.), 6, 45-57.

Perez-Martinez,L. Carreon-Rodriguez,A. Gonzalez-Alzati,M.E. Morales,C. Charli,J.L. Joseph-Bravo,P. 1998. Dexamethasone rapidly regulates TRH mRNA levels in hypothalamic cell cultures: interaction with the cAMP pathway Neuroendocrinology, 68, 345-354.

Sanchez,E. Charli,J.L. Morales,C. Corkidi,G. Seidah,N.G. Joseph-Bravo,P. Uribe,R.M. 1997. Expression of the proprotein convertases PC1 and PC2 mRNAs in thyrotropin releasing hormone neurons of the rat paraventricular nucleus of hypothalamus Brain Research, 761, 77-86.

Uribe,R.M. Perez-Martinez,L. Covarrubias,M.D. Gomez,O. Covarrubias,L. Charli,J.L. Joseph-Bravo,P. 1995. Phorbol ester or cAMP enhance thyrotropin-releasing hormone mRNA in primary cultures of hypothalamic cells Neuroscience Letters, 201, 41-44.

de Gortari,P. Fernandez-Guardiola,A. Martinez,A. Cisneros,M. Joseph-Bravo,P. 1995. Changes in TRH and its degrading enzyme pyroglutamyl peptidase II, during the development of amygdaloid kindling Brain Research, 679, 144-150.

Uribe,R.M. Joseph-Bravo,P. Charli,J.L. 1995. Pups removal enhances thyrotropin-releasing hormone mRNA in the hypothalamic paraventricular nucleus European Journal of Endocrinology, 133, 354-360.

Charli,J.L. Cruz,C. Redondo,J.L. Guerra,C. Joseph-Bravo,P. 1995. Homologous conditioned medium enhances expression of TRH in hypothalamic neurons in primary culture Developmental Brain Research, 89, 155-160.

Joseph-Bravo,P. 1994. Return of the native [3] Nature, 370, 408.

Covarrubias,L. Redondo,J.L. Vargas,M.A. Uribe,R.M. Mendez,M. Joseph-Bravo,P. Charli,J.L. 1994. In vitro TRH release from hypothalamus slices varies during the diurnal cycle Neurochemical Research, 19, 845-850.

Uribe,R.M. Joseph-Bravo,P. Ponce,G. Cisneros,M. Aceves,C. Charli,J.L. 1994. Influence of thyroid status on TRH metabolism in rat olfactory bulb Peptides, 15, 435-439.

Vargas,M.A. Joseph-Bravo,P. Charli,J.L. 1994. Thyrotropin-releasing hormone downregulates pyroglutamyl peptidase II activity in adenohypophyseal cells Neuroendocrinology, 60, 323-330.

Joseph-Bravo,P. Fresan,M. Cisneros,M. Vargas,M.A. Charli,J.L. 1994. Pyroglutamyl peptidase II activity is not in the processes of bulbospinal TRHergic neurons Neuroscience Letters, 178, 243-246.

Mendez,M. Moran,J. Wilk,S. Joseph-Bravo,P. Charli,J.L. 1993. Assessment of the role of TRH in the release of [3H]-dopamine from rat nucleus accumbens-lateral septum slices Brain Res.Bull, 31, 621-625.

Uribe,R.M. Redondo,J.L. Charli,J.L. Joseph-Bravo,P. 1993. Suckling and cold stress rapidly and transiently increase TRH mRNA in the paraventricular nucleus Neuroendocrinology, 58, 140-145.

Brakch,N. Boileau,G. Simonetti,M. Nault,C. Joseph-Bravo,P. Rholam,M. Cohen,P. 1993. Prosomatostatin processing in Neuro2A cells. Role of beta-turn structure in the vicinity of the Arg-Lys cleavage site European Journal of Biochemistry, 216, 39-47 * .

Vargas,M.A. Cisneros,M. Herrera,J. Joseph-Bravo,P. Charli,J.L. 1992. Regional distribution of pyroglutamyl peptidase II in rabbit brain, spinal cord, and organs Peptides, 13, 255-260.

Vargas,M.A. Herrera,J. Uribe,R.M. Charli,J.L. Joseph-Bravo,P. 1992. Ontogenesis of pyroglutamyl peptidase II activity in rat brain, adenohypophysis and pancreas Developmental Brain Research, 66, 251-256.

Uribe,R.M. Joseph-Bravo,P. Pasten,J. Ponce,G. Mendez,M. Covarrubias,L. Charli,J.L. 1991. Some events of thyrotropin-releasing hormone metabolism are regulated in lactating and cycling rats Neuroendocrinology, 54, 493-498.

Lepage-Lezin,A. Joseph-Bravo,P. Devilliers,G. Benedetti,L. Launay,J.M. Gomez,S. Cohen,P. 1991. Prosomatostatin is processed in the Golgi apparatus of rat neural cells Journal of Biological Chemistry, 266, 1679-1688 * .

Cruz,C. Charli,J.L. Vargas,M.A. Joseph-Bravo,P. 1991. Neuronal localization of pyroglutamate aminopeptidase II in primary cultures of fetal mouse brain Journal of Neurochemistry, 56, 1594-1601.

Fuchs,L.Y. Ovando,C. Joseph,P. Soberon,X. Charli,J.L. 1990. Repetitive DNA sequence from the crayfish Procambarus clarkii Nucleic Acids Research, 18, 1650.

Mendez,M. Cruz,C. Joseph-Bravo,P. Wilk,S. Charli,J.L. 1990. Evaluation of the role of prolyl endopeptidase and pyroglutamyl peptidase I in the metabolism of LHRH and TRH in brain Neuropeptides, 17, 55-62.

Charli,J.L. Mendez,M. Vargas,M.A. Cisneros,M. Assai,M. Joseph-Bravo,P. Wilk,S. 1989. Pyroglutamyl peptidase II inhibition specifically increases recovery of TRH released from rat brain slices Neuropeptides, 14, 191-196.

Covarrubias,L. Uribe,R.M. Mendez,M. Charli,J.L. Joseph-Bravo,P. 1989. Hypothalamic TRH mRNA regulation under different physiological conditions Annals of the New York Academy of Sciences, 553, 476-478.

Charli,J.L. Cruz,C. Ponce,G. Vargas,M.A. Mendez,M. Cisneros,M. Pasten,J. Joseph-Bravo,P. 1989. Physiological relevance of pyroglutamate aminopeptidase II Annals of the New York Academy of Sciences, 553, 563-564.

Charli,J.L. Cruz,C. Vargas,M. Joseph-Bravo,P. 1988. The narrow specificity pyroglutamate aminopeptidase degrading TRH in brain is an ectoenzyme Neurochemistry International, 13, 237-242.

Covarrubias,L. Uribe,R.M. Mendez,M. Charli,J.L. Joseph-Bravo,P. 1988. Neuronal TRH synthesis: developmental and circadian TRH mRNA levels Biochemical and Biophysical Research Communications, 151, 615-622.

Ponce,G. Charli,J.L. Pasten,J.A. Aceves,C. Joseph-Bravo,P. 1988. Tissue-specific regulation of pyroglutamate aminopeptidase II activity by thyroid hormones Neuroendocrinology, 48, 211-213.

Mendez,M. Joseph-Bravo,P. Cisneros,M. Vargas,M.A. Charli,J.L. 1987. Regional distribution of in vitro release of thyrotropin releasing hormone in rat brain Peptides, 8, 291-298.

Charli,J.L. Mendez,M. Joseph-Bravo,P. Wilk,S. 1987. Specific inhibitors of pyroglutamyl peptidase I and prolyl endopeptidase do not change the in vitro release of TRH or its content in rodent brain Neuropeptides, 9, 373-378.

Vargas,M. Mendez,M. Cisneros,M. Joseph-Bravo,P. Charli,J.L. 1987. Regional distribution of the membrane-bound pyroglutamate amino peptidase-degrading thyrotropin-releasing hormone in rat brain Neuroscience Letters, 79, 311-314.

Saavedra,R. Bravo,P.J. Charli,J.L. Herion,P. 1987. Characterization of high affinity monoclonal antibodies against the luteinizing hormone-releasing hormone Hybridoma, 6, 663-672.

Torres,H. Charli,J.L. Vargas,M.A. Gonzalez-Noriega,A. Joseph-Bravo,P. 1986. Subcellular distribution of the enzymes degrading Thyrotropin releasing hormone and metabolites in rat brain Neurochemistry International, 9, 103-110.

Cohen,S. Charli,J.L. Diaz de Leon,L. Millar,R.P. Arimura,A. Morrison,M.R. Joseph-Bravo,P. 1986. Attempts to immunoprecipitate the LHRH precursor synthesized in cell free systems Brain Research Bulletin, 16, 309-314.

Garat,B. Miranda,J. Charli,J.L. Joseph-Bravo,P. 1985. Presence of a membrane bound pyroglutamyl amino peptidase degrading thyrotropin releasing hormone in rat brain Neuropeptides, 6, 27-40.

Charli,J.L. Ponce,G. McKelvy,J.F. Joseph-Bravo,P. 1984. Accumulation of thyrotropin releasing hormone by rat hypothalamic slices Journal of Neurochemistry, 42, 981-986.

Pacheco,M.F. McKelvy,J.F. Woodward,D.J. Loudes,C. Joseph-Bravo,P. Krulich,L. Griffin,W.S. 1981. TRH in the rat cerebellum: I. Distribution and concentration Peptides, 2, 277-282 * .

Charli,J.L. Joseph-Bravo,P. Robert,M. 1980. Use of synthetic oligonucleotides for identification of messenger RNA for somatostatin.Boletin de estudios medicos y biologicos, 31, 220-221 * .

Joseph-Bravo,P. Charli,J.L. Sherman,T. Boyer,H. Bolivar,F. McKelvy,J.F. 1980. Identification of a putative hypothalamic mRNA coding for somatostatin and of its product in cell-free translation Biochemical and Biophysical Research Communications, 94, 1004-1012 * .

Joseph-Bravo,P. Charli,J.L. Palacios,J.M. Kordon,C. 1979. Effect of neurotransmitters on the in vitro release of immunoreactive thyrotropin-releasing hormone from rat mediobasal hypothalamus Endocrinology, 104, 801-806 * .

Joseph-Bravo,P. Loudes,C. Charli,J.L. Kordon,C. 1979. Subcellular distribution of brain peptidases degrading luteinizing hormone releasing hormone (LHRH) and thyrotropin releasing hormone (TRH) Brain Research, 166, 321-329 * .

Lin,C. Joseph-Bravo,P. Sherman,T. Chan,L. McKelvy,J.F. 1979. Cell-free synthesis of putative neurophysin precursors from rat and mouse hypothalamic poly (A)-RNA Biochemical and Biophysical Research Communications, 89, 943-950 * .

Loudes,C. Joseph-Bravo,P. Leblanc,P. Kordon,C. 1978. Specific activity of LHRH and TRH degrading enzymes in various tissues of normal and castrated male rats Biochemical and Biophysical Research Communications, 83, 921-926 * .

Charli,J.L. Joseph-Bravo,P. Palacios,J.M. Kordon,C. 1978. Histamine-induced release of thyrotropin releasing hormone from hypothalamic slices European Journal of Pharmacology, 52, 401-403 * .

Joseph-Bravo,P.I. Findley,M. Newberne,P.M. 1976. Some interactions of light, riboflavin, and aflatoxin B1 in vivo and in vitro Journal of Toxicology and Environmental Health, 1, 353-376 * .

Libros y capítulos recientes

Joseph-Bravo,P. Gutierrez-Mariscal,M. Jaimes-Hoy,L. Charli,J.L. 2019. Thyroid Axis and Energy Balance: Focus on Animals and Implications for Humankind en: Patel,V.B. Handbook of Famine, Starvation, and Nutrient Deprivation: From Biology to Policy. Cham, Suiza. Springer International Publishing. pags. 1099-1126

de Gortari,P. Joseph-Bravo,P. 2010. Regulacion neuroendocrina del apetito en: Morales-Gonzalez,J.A. Obesidad. Un enfoque multidisciplinario. Pachuca. Universidad Autonoma del Edo. de Hidalgo. pags. 57-71

Joseph-Bravo,P. de Gortari,P. 2008. El estrés y sus efectos en el metabolismo y el aprendizaje en: *agustin Una ventana al quehacer científico. Instituto de Biotecnología de la UNAM 25 aniversario, cap 6. Mexico, D.F.. UNAM. pags. 65-76

Charli,J.L. Joseph-Bravo,P. 2008. El cerebro, la comunicación intercelular y los péptidos en: *agustin Una ventana al quehacer científico. Instituto de Biotecnología de la UNAM 25 aniversario, cap 5. Mexico, D.F.. UNAM. pags. 53-64

Joseph-Bravo,P. Mariscal,M. Garcia,I. Jaimes-Hoy,L. Charli,J.L. de Gortari,P. 2007. Response of limbic TRH neurons depends on the stress paradigm en: Endocrine Regulations. Int. Congress of Stress. 96-

de Gortari,P. Joseph,P. 2006. Neuroendocrine Regulation of Energy Metabolism en: *joseph Molecular Endocrinology. Kerala, India,. Research Signpost. pags. 65-86

Joseph-Bravo,P. Cote-Velez,A. Perez-Martinez,L. 2006. Integration of Neuroendocrine Signals that Regulate the Activity of Hypophysiotropic Peptides en: *joseph Molecular Endocrinology. Kerala, India,. Research Signpost. pags. 1-24

Joseph-Bravo,P. 2006. Molecular Endocrinology Kerala, India:. Research Signpost.

Joseph-Bravo,P. 2005. Choques, transtornos y otras caídas. Stress, dolor y péptidos. en: Tapia,R. Memoria del Curso Explorando el Cerebro.. Mexico,D.F.. Universum e Instituto de Fisiologia Celular. pags. 35-37

Joseph-Bravo,P. 1995. Choques, transtornos y otras caídas. Stress, dolor y péptidos en: Memoria del Curso Explorando el Cerebro. Mexico,D.F.. Universum e Instituto de Fisiologia Celular. pags. 35-37

Charli,J.L. Garat,B. Martinez-Escalera,G. Ponce,G. Miranda,J. Joseph-Bravo,P. 1984. METABOLISM OF THYROTROPIN RELEASING HORMONE AND ITS POSSIBLE RELEVANCE TO PROLACTIN SECRETION en: Valverde,R. Prolactin Secretion: a Multidisciplinary Approach. Academic Press. pags. 239-248

Kordon,C. Enjalbert,A. Hery,M. Joseph-Bravo,P. Rotsztjen,W. Ruberg.M. 1979. Role of neurotransmitters in the control of adenohypophyseal secretion en: Panksepp,J. Handbook of the hypothalamus. New York. Dekker. pags. 253-306 *

McKelvy,J.F. Lin,C. Chan,L. Joseph-Bravo,P. Charli,J.L. Pacheco,M. 1979. Biosynthesis of brain peptides en: Boyd,A.E. Amsterdam ; New York. Elsevier/North-Holland Biomedical Press. pags. 183-196 *

Otras Publicaciones

Carreon-Rodriguez,A. Perez-Martinez,L. Joseph-Bravo,P. 2008. La participación de las hormonas tiroideas en el desarrollo y función del sistema nervioso.Facultad de Medicina.Revista Actualidades de Posgrado para el Médico General, 12, 9-12.

Charli,J.L. Ponce,G. Torres,H. Garat,B. Barquin,N. Bravo,P.J. 1983. [Cellular biochemistry of hypophysiotropic peptides. II. Liberation, action and inactivation].Boletin de estudios medicos y biologicos, 32, 243-252.

Joseph-Bravo,P. Theleen,M. de Gortari,P. Shapiro,E. Redondo,J.L. Briones,M. Merchant,H. Charli,J.L. 1983. [Biochemistry of hypophysiotropic peptides. I. Biosynthesis and its regulation].Boletin de estudios medicos y biologicos, 32, 233-241.

* Indica publicación con otra institución de adscripción