. Instituto de Biotecnologia UNAM

IBT-UNAM : M.B. Timoteo Celso Olamendi Portugal

M.B. Timoteo Celso Olamendi Portugal

Técnico Académico
Nivel I del SNI
Grupo del Dr. Baltazar Becerril

email: timoteo @ibt.unam.mx
Teléfonos
Oficina +52 (777) 311-4900 ext 247

Laboratorio +52 (777) 329-1669
desde la CDMX 562-27669
red UNAM 27669
+52 (777) 311-4900 ext 247

Publicaciones recientes

Alvarado,D. Cardoso-Arenas,S. Corrales-Garcia,L.L. Clement,H. Arenas,I. Montero-Dominguez,P.A. Olamendi-Portugal,T. Zamudio,F. Agota,C. Borrego,J. Panyi,G. Papp,F. Corzo,G. 2021. A Novel Insecticidal Spider Peptide that Affects the Mammalian Voltage-Gated Ion Channel hKv1.5 Frontiers in Pharmacology, 11, 563858.

Lopez-Giraldo,A.E. Olamendi-Portugal,T. Riano-Umbarila,L. Becerril,B. Possani,L.D. Delepierre,M. Rio-Portilla,F.D. 2020. The three-dimensional structure of the toxic peptide Cl13 from the scorpion Centruroides limpidus Toxicon, 184, 158-166.

Gomez-Ramirez,I.V. Riano-Umbarila,L. Olamendi-Portugal,T. Restano-Cassulini,R. Possani,L.D. Becerril,B. 2020. Biochemical, electrophysiological and immunological characterization of the venom from Centruroides baergi, a new scorpion species of medical importance in Mexico Toxicon, 184, 10-18.

Riano-Umbarila,L. Rojas-Trejo,V.M. Romero-Moreno,J.A. Costas,M. Utrera-Espindola,I. Olamendi-Portugal,T. Possani,L.D. Becerril,B. 2020. Comparative assessment of the VH-VL and VL-VH orientations of single-chain variable fragments of scorpion toxin-neutralizing antibodies Molecular Immunology, 122, 141-147.

Valdez-Velazquez,L.L. Cid-Uribe,J. Romero-Gutierrez,M.T. Olamendi-Portugal,T. Jimenez-Vargas,J.M. Possani,L.D. 2020. Transcriptomic and proteomic analyses of the venom and venom glands of Centruroides hirsutipalpus, a dangerous scorpion from Mexico Toxicon, 179, 21-32.

Rincon-Cortes,C.A. Olamendi-Portugal,T. Carcamo-Noriega,E.N. Gonzalez-Santillan,E. Zamudio-Zuniga,F. Reyes-Montano,E.A. Vega Castro,N.A. Possani,L.D. 2019. Structural and functional characterization of toxic peptides purified from the venom of the Colombian scorpion Tityus macrochirus Toxicon, 169, 5-11.

Riano-Umbarila,L. Gomez-Ramirez,I.V. Ledezma-Candanoza,L.M. Olamendi-Portugal,T. Rodriguez-Rodriguez,E.R. Fernandez-Taboada,G. Possani,L.D. Becerril,B. 2019. Generation of a Broadly Cross-Neutralizing Antibody Fragment against Several Mexican Scorpion Venoms Toxins (Basel), 11, 32.

Olamendi-Portugal,T. Batista,C.V.F. Pedraza-Escalona,M. Restano-Cassulini,R. Zamudio,F.Z. Benard-Valle,M. de Roodt,A.R. Possani,L.D. 2018. New insights into the proteomic characterization of the coral snake Micrurus pyrrhocryptus venom Toxicon, 153, 23-31.

Valdez-Velazquez,L.L. Olamendi-Portugal,T. Restano-Cassulini,R. Zamudio,F.Z. Possani,L.D. 2018. Mass fingerprinting and electrophysiological analysis of the venom from the scorpion Centruroides hirsutipalpus (Scorpiones: Buthidae) Journal of Venomous Animals and Toxins Including Tropical Diseases, 24, 17.

Carcamo-Noriega,E.N. Olamendi-Portugal,T. Restano-Cassulini,R. Rowe,A. Uribe-Romero,S.J. Becerril,B. Possani,L.D. 2018. Intraspecific variation of Centruroides sculpturatus scorpion venom from two regions of Arizona Archives of Biochemistry and Biophysics, 638, 52-57.

Garcia-Garcia,F. Corrales-Garcia,L.L. Olamendi-Portugal,T. Restano-Cassulini,R. Vega,R. Vega,M. Ortiz,E. Sandoval-Lopez,G. Soto,E. Corzo,G. 2017. Identification, chemical synthesis and heterologous expression of an antinociceptive peptide from the veined tree frog Trachycephalus typhonius Process Biochemistry, 62, 205-214.

Olamendi-Portugal,T. Csoti,A. Jimenez-Vargas,J.M. Gomez-Lagunas,F. Panyi,G. Possani,L.D. 2017. Pi5 and Pi6, two undescribed peptides from the venom of the scorpion Pandinus imperator and their effects on K+-channels Toxicon, 133, 136-144.

Olamendi-Portugal,T. Restano-Cassulini,R. Riano-Umbarila,L. Becerril,B. Possani,L.D. 2017. Functional and immuno-reactive characterization of a previously undescribed peptide from the venom of the scorpion centruroides limpidus Peptides, 87, 34-40.

Rodriguez-Rodriguez,E.R. Olamendi-Portugal,T. Serrano-Posada,H. Arredondo-Lopez,J.N. Gomez-Ramirez,I. Fernandez-Taboada,G. Possani,L.D. Vega,G.A. Riano-Umbarila,L. Becerril,B. 2016. Broadening the neutralizing capacity of a family of antibody fragments against different toxins from Mexican scorpions Toxicon, 119, 52-63.

Olamendi-Portugal,T. Bartok,A. Zamudio-Zuniga,F. Balajthy,A. Becerril,B. Panyi,G. Possani,L.D. 2016. Isolation, chemical and functional characterization of several new K-channel blocking peptides from the venom of the scorpion Centruroides tecomanus Toxicon, 115, 1-12.

Riano-Umbarila,L. Ledezma-Candanoza,L.M. Serrano-Posada,H. Fernandez-Taboada,G. Olamendi-Portugal,T. Rojas-Trejo,S. Gomez-Ramirez,I.V. Rudino-Pinera,E. Possani,L.D. Becerril,B. 2016. Optimal neutralization of Centruroides noxius venom is understood through a structural complex between two antibody fragments and the Cn2 toxin Journal of Biological Chemistry, 291, 1619-1630.

Villalba,M.I. Canul-Tec,J.C. Luna-Martinez,O.D. Sanchez-Alcala,R. Olamendi-Portugal,T. Rudino-Pinera,E. Rojas,S. Sanchez-Lopez,R. Fernandez-Velasco,D.A. Becerril,B. 2015. Site-directed Mutagenesis Reveals Regions Implicated in the Stability and Fiber Formation of Human lambda3r Light Chains Journal of Biological Chemistry, 290, 2577-2592 [Correction vol 290 p 13039].

Riano-Umbarila,L. Olamendi-Portugal,T. Morelos-Juarez,C. Gurrola,G.B. Possani,L.D. Becerril,B. 2013. A novel human recombinant antibody fragment capable of neutralizing Mexican scorpion toxins Toxicon, 76, 370-376.

Rodriguez-Rodriguez,E.R. Ledezma-Candanoza,L.M. Contreras-Ferrat,L.G. Olamendi-Portugal,T. Possani,L.D. Becerril,B. Riano-Umbarila,L. 2012. A Single Mutation in Framework 2 of the Heavy Variable Domain Improves the Properties of a Diabody and a Related Single-Chain Antibody Journal of Molecular Biology, 423, 337-350.

Schiavon,E. Pedraza-Escalona,M. Gurrola,G.B. Olamendi-Portugal,T. Corzo,G. Wanke,E. Possani,L.D. 2012. Negative-shift activation, current reduction and resurgent currents induced by beta-toxins from Centruroides scorpions in sodium channels Toxicon, 59, 283-293.

Olivares,J.E. Diaz-Camino,C. Estrada-Navarrete,G. Alvarado-Affantranger,X. Rodriguez-Kessler,M. Zamudio,F.Z. Olamendi-Portugal,T. Marquez,Y. Servin,L.E. Sanchez,F. 2011. Nodulin 41, a novel late nodulin of common bean with peptidase activity BMC Plant Biology, 11, 134.

Espino-Solis,G.P. Estrada,G. Olamendi-Portugal,T. Villegas,E. Zamudio,F. Cestele,S. Possani,L.D. Corzo,G. 2011. Isolation and molecular cloning of beta-neurotoxins from the venom of the scorpion Centruroides suffusus suffusus Toxicon, 57, 739-746.

Riano-Umbarila,L. Contreras-Ferrat,G. Olamendi-Portugal,T. Morelos-Juarez,C. Corzo,G. Possani,L.D. Becerril,B. 2011. Exploiting cross-reactivity to neutralize two different scorpion venoms with one single-chain antibody fragment Journal of Biological Chemistry, 286, 6143-6151.

Vandendriessche,T. Olamendi-Portugal,T. Zamudio,F.Z. Possani,L.D. Tytgat,J. 2010. Isolation and characterization of two novel scorpion toxins: the alpha-toxin-like CeII8, specific for Na(v)1.7 channels and the classical anti-mammalian CeII9, specific for Na(v)1.4 channels Toxicon, 56, 613-623.

Garcia-Gomez,B.I. Olamendi-Portugal,T.C. Paniagua,J. van der Walt,J. Dyason,K. Possani,L.D. 2009. Heterologous expression of a gene that codes for Pg8, a scorpion toxin of Parabuthus granulatus, capable of generating protecting antibodies in mice Toxicon, 53, 770-778.

Olamendi-Portugal,T. Batista,C.V. Restano-Cassulini,R. Pando,V. Villa-Hernandez,O. Zavaleta-Martinez-Vargas,A. Salas-Arruz,M.C. de la Vega RC Becerril,B. Possani,L.D. 2008. Proteomic analysis of the venom from the fish eating coral snake Micrurus surinamensis: Novel toxins, their function and phylogeny Proteomics, 8, 1919-1932.

Restano-Cassulini,R. Olamendi-Portugal,T. Zamudio,F. Becerril,B. Possani,L.D. 2008. Two Novel Ergtoxins, Blockers of K(+)-channels, Purified from the Mexican Scorpion Centruroides elegans elegans Neurochemical Research, 33, 1525-1533.

Olamendi-Portugal,T. Somodi,S. Fernandez,J.A. Zamudio,F.Z. Becerril,B. Varga,Z. Panyi,G. Gaspar,R. Possani,L.D. 2005. Novel alpha-KTx peptides from the venom of the scorpion Centruroides elegans selectively blockage Kv1.3 over IKCa1 K(+) channels of T cells Toxicon, 46, 418-429.

Riano-Umbarila,L. Juarez-Gonzalez,V.R. Olamendi-Portugal,T. Ortiz-Leon,M. Possani,L.D. Becerril,B. 2005. A strategy for the generation of specific human antibodies by directed evolution and phage display Febs Journal, 272, 2591-2601.

Juarez-Gonzalez,V.R. Riano-Umbarila,L. Quintero-Hernandez,V. Olamendi-Portugal,T. Ortiz-Leon,M. Ortiz,E. Possani,L.D. Becerril,B. 2005. Directed Evolution, Phage Display and Combination of Evolved Mutants: A Strategy to Recover the Neutralization Properties of the scFv Version of BCF2 a Neutralizing Monoclonal Antibody Specific to Scorpion Toxin Cn2 Journal of Molecular Biology, 346, 1287-1297.

Gomez-Lagunas,F. Batista,C.V. Olamendi-Portugal,T. Ramirez-Dominguez,M.E. Possani,L.D. 2004. Inhibition of the collapse of the shaker k+ conductance by specific scorpion toxins Journal of General Physiology, 123, 265-279.

Huys,I. Olamendi-Portugal,T. Garcia-Gomez,B.I. Vandenberghe,I. Van Beeumen,J. Dyason,K. Clynen,E. Zhu,S. van der Walt,J. Possani,L. Tytgat,J. 2004. A subfamily of acidic alpha -K+ toxins Journal of Biological Chemistry, 279, 2781-2789.

M'Barek,S. Mosbah,A. Sandoz,G. Fajloun,Z. Olamendi-Portugal,T. Rochat,H. Sampieri,F. Guijarro,J.I. Mansuelle,P. Delepierre,M. De Waard,M. Sabatier,J.M. 2003. Synthesis and characterization of Pi4, a scorpion toxin from Pandinus imperator that acts on K+ channels European Journal of Biochemistry, 270, 3583-3592.

Coronas,F.V. de Roodt,A.R. Portugal,T.O. Zamudio,F.Z. Batista,C.V. Gomez-Lagunas,F. Possani,L.D. 2003. Disulfide bridges and blockage of Shaker B K(+)-channels by another butantoxin peptide purified from the Argentinean scorpion Tityus trivittatus Toxicon, 41, 173-179.

Lopez-Gonzalez,I. Olamendi-Portugal,T. de la Vega-Beltran,J.L. van der Walt,J. Dyason,K. Possani,L.D. Felix,R. Darszon,A. 2003. Scorpion toxins that block T-type Ca(2+) channels in spermatogenic cells inhibit the sperm acrosome reaction Biochemical and Biophysical Research Communications, 300, 408-414.

Angulo,Y. Olamendi-Portugal,T. Alape-Giron,A. Possani,L.D. Lomonte,B. 2002. Structural characterization and phylogenetic relationships of myotoxin II from Atropoides (Bothrops) nummifer snake venom, a Lys49 phospholipase A(2) homologue International Journal of Biochemistry and Cell Biology, 34, 1268-1278.

Olamendi-Portugal,T. Garcia,B.I. Lopez-Gonzalez,I. van der Walt,J. Dyason,K. Ulens,C. Tytgat,J. Felix,R. Darszon,A. Possani,L.D. 2002. Two new scorpion toxins that target voltage-gated Ca(2+) and Na(+) channels Biochemical and Biophysical Research Communications, 299, 562-568.

Ramirez-Dominguez,M.E. Olamendi-Portugal,T. Garcia,U. Garcia,C. Arechiga,H. Possani,L.D. 2002. Cn11, the first example of a scorpion toxin that is a true blocker of Na(+) currents in crayfish neurons Journal of Experimental Biology, 205, 869-876.

Angulo,Y. Olamendi-Portugal,T. Possani,L.D. Lomonte,B. 2000. Isolation and characterization of myotoxin II from Atropoides (Bothrops) nummifer snake venom, a new Lys49 phospholipase A2 homologue International Journal of Biochemistry and Cell Biology, 32, 63-71.

Batista,C.V. da Silva,L.R. Sebben,A. Scaloni,A. Ferrara,L. Paiva,G.R. Olamendi-Portugal,T. Possani,L.D. Bloch,C. 1999. Antimicrobial peptides from the Brazilian frog Phyllomedusa distincta Peptides, 20, 679-686.

Olamendi-Portugal,T. Gomez-Lagunas,F.G. Gurrola,G.B. Possani,L.D. 1998. Two similar peptides from the venom of the scorpion Pandinus imperator, one highly effective blocker and the other inactive on K+ channels Toxicon, 36, 759-770.

Gomez-Lagunas,F. Olamendi-Portugal,T. Possani,L.D. 1997. Block of ShakerB K+ channels by Pi1, a novel class of scorpion toxin FEBS Letters, 400, 197-200.

Olamendi-Portugal,T. Gomez-Lagunas,F. Gurrola,G.B. Possani,L.D. 1996. A novel structural class of K+-channel blocking toxin from the scorpion Pandinus imperator Biochemical Journal, 315, 977-981.

Gomez-Lagunas,F. Olamendi-Portugal,T. Zamudio,F.Z. Possani,L.D. 1996. Two novel toxins from the venom of the scorpion Pandinus imperator show that the N-terminal amino acid sequence is important for their affinities towards Shaker B K+ channels Journal of Membrane Biology, 152, 49-56.

Calderon-Aranda,E.S. Olamendi-Portugal,T. Possani,L.D. 1995. The use of synthetic peptides can be a misleading approach to generate vaccines against scorpion toxins Vaccine, 13, 1198-1206.

Possani,L.D. Gurrola,G.B. Portugal,T.O. Zamudio,F.Z. Vaca,L.D. Calderon,S.A. Kirsch,G.E. 1991. Scorpion toxins: a model for peptide synthesis of new drugs en: Sgarbieri,V. Proceedings First Brazilian Congress on Proteins. Brasil. UNICAMP. pags. 352-367

Patentes

Corzo, G., Garcia, F., Olamendi, T., Sandoval, G., 2015 Proteina analgésica aislada de la secreción de la rana Trachycephalous venulosus. Oficina de Patentes Mexico. (en trámite)