. Instituto de Biotecnologia UNAM

IBT-UNAM : Dr. Octavio Tonatiuh Ramirez Reivich

Dr. Octavio Tonatiuh Ramirez Reivich

Director
Líder Académico »» Grupo
Investigador
Tutor de Maestría y Doctorado
Nivel III del SNI
Líneas de investigación

email: tonatiuh @ibt.unam.mx
Teléfonos
Oficina (52+55) 562-27671 ; (52+55) 562-27672 ; +52 (777) 317-2399

Oficina +52 (777) 329-1646
desde la CDMX 562-27646
red UNAM 27646
+52 (777) 311-4900 ext 206

Laboratorio +52 (777) 329-1617
desde la CDMX 562-27617
red UNAM 27617
+52 (777) 311-4900 ext 206

- Licenciatura: Ingeniero Químico, Fac. de Química-UNAM (1985)
- Maestría: Ingeniero Químico, Drexel University, Pensilvania, E.U.A. (1987)
- Doctorado: Ingeniero Químico, Drexel University, Pensilvania, E.U.A. (1990)
- Mencion honorífica en examen de Licenciatura (1985)
- Premio por merito academico al mejor estudiante internacional, Universidad de Drexel (1989 y 1990)
- Premio Sigma al mejor trabajo de investigacion de posgrado, otorgado durante la Semana Nacional de Ciencia y Tecnología, Universidad de Drexel, Filadelfia, E.U.A. (1990)

Premio Universidad Nacional 2010 en el área de Innovación Tecnológica y Diseño Industrial UNAM (2010)
Premio Canifarma Veterinaria. 1er lugar en la modalidad de desarrollo tecnológico (2010)
Premio Canifarma Veterinaria. 1er lugar en la modalidad de desarrollo tecnológico.Trabajo Desarrollo de una vacuna recombinante contra rotavirus bovinos prevalecientes en México (2009)
Distinción Universidad Nacional para Jóvenes Académicos en el área de Innovación Tecnológica y Diseño Industrial (2000)
Premio Nacional de Tecnología (1999)
Miembro de la Academia Mexicana de Ciencias (1999)
Premio de la Academia Mexicana de Ciencias en el área de Investigación Tecnológica (1998)
Premio Carlos Casas Campillo Sociedad Mexicana de Biotecnologia y Bioingenieria A.C. (1996)

Estudiantes

Ing. Emmanuel Pichardo

Lic. Itandehui Alheli Lopez

Q.F.B. Yaice Berenice Sandoval

I.B.Q. Victor Manuel López

M.C. Dubhe Beatriz Bulte

Publicaciones recientes

Villanueva-Flores,F. Castro-Lugo,A. Ramirez,O. Palomares,L.A. 2020. Understanding cellular interactions with nanomaterials: Towards a rational design of medical nanodevices Nanotechnology, 31, 132002.

Fernandez-Sandoval,M.T. Galindez-Mayer,J. Bolivar,F. Gosset,G. Ramirez,O.T. Martinez,A. 2019. Xylose-glucose co-fermentation to ethanol by Escherichia coli strain MS04 using single- and two-stage continuous cultures under micro-aerated conditions Microbial Cell Factories, 18, 145.

Pastor,A.R. Gonzalez-Dominguez,G. Diaz-Salinas,M.A. Ramirez,O.T. Palomares,L.A. 2019. Defining the multiplicity and time of infection for the production of Zaire Ebola virus-like particles in the insect cell-baculovirus expression system Vaccine, 37, 6962-6969.

Garcia-Garcia,W.I. Vidal-Limon,A. Arrocha-Arcos,A.A. Palomares,L.A. Ramirez,O.T. Miranda-Hernandez,M. 2019. Rotavirus VP6 protein as a bio-electrochemical scaffold: Molecular dynamics and experimental electrochemistry Bioelectrochemistry, 127, 180-186.

Villanueva-Flores,F. Miranda-Hernandez,M. Flores-Flores,J.O. Porras-Sanjuanico,A. Hu,H. Perez-Martinez,L. Ramirez,O.T. Palomares,L.A. 2019. Poly(vinyl alcohol co-vinyl acetate) as a novel scaffold for mammalian cell culture and controlled drug release Journal of Materials Science, 54, 7867-7882.

Martinez,J.A. Rodriguez,A. Moreno,F. Flores,N. Lara,A.R. Ramirez,O.T. Gosset,G. Bolivar,F. 2018. Metabolic modeling and response surface analysis of an Escherichia coli strain engineered for shikimic acid production BMC Systems Biology, 12, 102.

Gomez,I.A. Hernandez,V. Palomares,L.A. Ramirez,O.T. 2018. Flocculation of CHO cells for primary separation of recombinant glycoproteins: Effect on glycosylation profiles Biochemical Engineering Journal, 132, 244-254.

Hidalgo,D. Paz,E. Palomares,L.A. Ramirez,O.T. 2017. Real-time imaging reveals unique heterogeneous population features in insect cell cultures Journal of Biotechnology, 259, 56-62.

Pablos,T.E. Olivares,R. Sigala,J.C. Ramirez,O.T. Lara,A.R. 2016. Toward efficient microaerobic processes using engineered Escherichia coli W3110 strains Engineering in Life Sciences, 16, 588-597.

Carreno-Fuentes,L. Bahena,D. Palomares,L.A. Ramirez,O.T. Jose-Yacaman,M. Plascencia-Villa,G. 2016. Molecular docking and aberration-corrected STEM of palladium nanoparticles on viral templates Metals, 6, .

Bedoya-Lopez,A. Estrada,K. Sanchez-Flores,A. Ramirez,O.T. Altamirano,C. Segovia,L. Miranda-Rios,J. Trujillo-Roldan,M.A. Valdez-Cruz,N.A. 2016. Effect of Temperature Downshift on the Transcriptomic Responses of Chinese Hamster Ovary Cells Using Recombinant Human Tissue Plasminogen Activator Production Culture PLoS ONE, 11, e0151529.

Cortes,J.T. Flores,N. Bolivar,F. Lara,A.R. Ramirez,O.T. 2016. Physiological effects of pH gradients on Escherichia coli during plasmid DNA production Biotechnology and Bioengineering, 113, 598-611.

Carreno-Fuentes,L. Plascencia-Villa,G. Palomares,L.A. Moya,S.E. Ramirez,O.T. 2014. Modulating the physicochemical and structural properties of gold-functionalized protein nanotubes through thiol surface modification Langmuir, 30, 14991-14998.

Plascencia-Villa,G. Carreno-Fuentes,L. Bahena,D. Jose-Yacaman,M. Palomares,L.A. Ramirez,O.T. 2014. Characterization of conductive nanobiomaterials derived from viral assemblies by low-voltage STEM imaging and Raman scattering Nanotechnology, 25, 385706.

Rodriguez-Limas,W.A. Pastor,A.R. Esquivel-Soto,E. Esquivel-Guadarrama,F. Ramirez,O.T. Palomares,L.A. 2014. Immunogenicity and protective efficacy of yeast extracts containing rotavirus-like particles: A potential veterinary vaccine Vaccine, 32, 2794-2798.

Pastor,A.R. Rodriguez-Limas,W.A. Contreras,M.A. Esquivel,E. Esquivel-Guadarrama,F. Ramirez,O.T. Palomares,L.A. 2014. The assembly conformation of rotavirus VP6 determines its protective efficacy against rotavirus challenge in mice Vaccine, 32, 2874-2877.

Castro-Acosta,R.M. Rodriguez-Limas,W.A. Valderrama,B. Ramirez,O.T. Palomares,L.A. 2014. Effect of metal catalyzed oxidation in recombinant viral protein assemblies Microbial Cell Factories, 13, 25.

Rodriguez,M. Wood,C. Sanchez-Lopez,R. Castro-Acosta,R.M. Ramirez,O.T. Palomares,L.A. 2014. Understanding internalization of rotavirus VP6 nanotubes by cells: towards a recombinant vaccine Archives of Virology, 159, 1005-1015.

Wunderlich,M. Taymaz-Nikerel,H. Gosset,G. Ramirez,O.T. Lara,A.R. 2014. Effect of growth rate on plasmid DNA production and metabolic performance of engineered Escherichia coli strains Journal of Bioscience and Bioengineering, 117, 336-342.

Rodriguez,A. Martinez,J.A. Baez-Viveros,J.L. Flores,N. Hernandez-Chavez,G. Ramirez,O.T. Gosset,G. Bolivar,F. 2013. Constitutive expression of selected genes from the pentose phosphate and aromatic pathways increases the shikimic acid yield in high-glucose batch cultures of an Escherichia coli strain lacking PTS and pykF Microbial Cell Factories, 12, 86.

Jaen,K.E. Lara,A.R. Ramirez,O.T. 2013. Effect of heating rate on pDNA production by E. coli Biochemical Engineering Journal, 79, 230-238.

Plascencia-Villa,G. Medina,A. Palomares,L.A. Ramirez,O.T. Ascencio,J.A. 2013. Structural Characterization of Rotavirus-Directed Synthesis and Assembly of Metallic Nanoparticle Arrays Journal of Nanoscience and Nanotechnology, 13, 5572-5579.

Caspeta,L. Lara,A.R. Perez,N.O. Flores,N. Bolivar,F. Ramirez,O.T. 2013. Enhancing thermo-induced recombinant protein production in Escherichia coli by temperature oscillations and post-induction nutrient feeding strategies Journal of Biotechnology, 167, 47-55.

Carreno-Fuentes,L. Ascencio,J.A. Medina,A. Aguila,S. Palomares,L.A. Ramirez,O.T. 2013. Strategies for specifically directing metal functionalization of protein nanotubes: constructing protein coated silver nanowires Nanotechnology, 24, 235602.

Fuentes,L.G. Lara,A.R. Martinez,L.M. Ramirez,O.T. Martinez,A. Bolivar,F. Gosset,G. 2013. Modification of glucose import capacity in Escherichia coli: physiologic consequences and utility for improving DNA vaccine production Microbial Cell Factories, 12, 42.

Meneses-Acosta,A. Vizcaino-Meza,L.R. Ayala-Castro,H.G. Contreras,M.A. Ortega-Lopez,J. Ramirez,O.T. 2013. Effect of controlled redox potential and dissolved oxygen on the in vitro refolding of an E. coli alkaline phosphatase and chicken lysozyme Enzyme and Microbial Technology, 52, 312-318.

Borja,G.M. Meza-Mora E. Barron,B. Gosset,G. Ramirez,O.T. Lara,A.R. 2012. Engineering Escherichia coli to increase plasmid DNA production in high cell-density cultivations in batch mode Microbial Cell Factories, 11, 132.

Meneses-Acosta,A. Gomez,A. Ramirez,O.T. 2012. Control of redox potential in hybridoma cultures: effects on MAb production, metabolism, and apoptosis Journal of Industrial Microbiology & Biotechnology, 39, 1189-1198.

Martinez-Gomez,K. Flores,N. Castaneda,H.M. Martinez-Batallar,G. Hernandez-Chavez,G. Ramirez,O.T. Gosset,G. Encarnacion,S. Bolivar,F. 2012. New insights into Escherichia coli metabolism: carbon scavenging, acetate metabolism and carbon recycling responses during growth on glycerol Microbial Cell Factories, 11, 46.

Palomares,L.A. Mena,J.A. Ramirez,O.T. 2012. Simultaneous expression of recombinant proteins in the insect cell-baculovirus system: Production of virus-like particles Methods, 56, 389-395.

Pablos,T.E. Soto,R. Mora,E.M. Le Borgne,S. Ramirez,O.T. Gosset,G. Lara,A.R. 2012. Enhanced production of plasmid DNA by engineered Escherichia coli strains Journal of Biotechnology, 158, 211-214.

Lara,A.R. Knabben,I. Regestein,L. Sassi,J. Caspeta,L. Ramirez,O.T. Buchs,J. 2011. Comparison of oxygen enriched air vs. pressure cultivations to increase oxygen transfer and to scale-up plasmid DNA production fermentations Engineering in Life Sciences, 11, 382-386.

Soto,R. Caspeta,L. Barron,B. Gosset,G. Ramirez,O.T. Lara,A.R. 2011. High cell-density cultivation in batch mode for plasmid DNA production by a metabolically engineered E. coli strain with minimized overflow metabolism Biochemical Engineering Journal, 56, 165-171.

Pablos,T.E. Mora,E.M. Le Borgne,S. Ramirez,O.T. Gosset,G. Lara,A.R. 2011. Vitreoscilla hemoglobin expression in engineered Escherichia coli: Improved performance in high cell-density batch cultivations Biotechnology Journal, 6, 993-1002.

Baez,A. Flores,N. Bolivar,F. Ramirez,O.T. 2011. Simulation of dissolved CO(2) gradients in a scale-down system: A metabolic and transcriptional study of recombinant Escherichia coli Biotechnology Journal, 6, 959-967.

Valdez-Cruz,N.A. Ramirez,O.T. Trujillo-Roldan,M.A. 2011. Molecular responses of Escherichia coli caused by heat stress and recombinant protein production during temperature induction Bioengineered Bugs, 2, 105-110.

Rodriguez-Limas,W.A. Tyo,K.E. Nielsen,J. Ramirez,O.T. Palomares,L.A. 2011. Molecular and process design for rotavirus-like particle production in Saccharomyces cerevisiae Microbial Cell Factories, 10, 33.

Plascencia-Villa,G. Mena,J.A. Castro-Acosta,R.M. Fabian,J.C. Ramirez,O.T. Palomares,L.A. 2011. Strategies for the purification and characterization of protein scaffolds for the production of hybrid nanobiomaterials Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 879, 1105-1111.

Gallo-Ramirez,L.E. Ramirez,O.T. Palomares,L.A. 2011. Intracellular localization of adeno-associated viral proteins expressed in insect cells Biotechnology Progress, 27, 483-493.

De Leon-Rodriguez,A. Galindo,E. Ramirez,O.T. 2010. Design and characterization of a one-compartment scale-down system for simulating dissolved oxygen tension gradients Journal of Chemical Technology and Biotechnology, 85, 950-956.

Escalante,A. Calderon,R. Valdivia,A. de Anda,R. Hernandez,G. Ramirez,O.T. Gosset,G. Bolivar,F. 2010. Metabolic engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system Microbial Cell Factories, 9, 21.

Castro-Acosta,R.M. Revilla,A.L. Ramirez,O.T. Palomares,L.A. 2010. Separation and quantification of double- and triple-layered rotavirus-like particles by CZE Electrophoresis, 31, 1376-1381.

Valdez-Cruz,N.A. Caspeta,L. Perez,N.O. Ramirez,O.T. Trujillo-Roldan,M.A. 2010. Production of recombinant proteins in E. coli by the heat inducible expression system based on the phage lambda pL and/or pR promoters Microbial Cell Factories, 9, 18.

Lara,A.R. Taymaz-Nikerel,H. Mashego,M.R. van Gulik,W.M. Heijnen,J.J. Ramirez,O.T. van Winden,W.A. 2009. Fast dynamic response of the fermentative metabolism of Escherichia coli to aerobic and anaerobic glucose pulses Biotechnology and Bioengineering, 104, 1153-1161.

Plascencia-Villa,G. Saniger,J.M. Ascencio,J.A. Palomares,L.A. Ramirez,O.T. 2009. Use of recombinant rotavirus VP6 nanotubes as a multifunctional template for the synthesis of nanobiomaterials functionalized with metals Biotechnology and Bioengineering, 104, 871-881.

Mellado,M.C. Mena,J.A. Lopes,A. Ramirez,O.T. Carrondo,M.J. Palomares,L.A. Alves,P.M. 2009. Impact of physicochemical parameters on in vitro assembly and disassembly kinetics of recombinant triple-layered rotavirus-like particles Biotechnology and Bioengineering, 104, 674-686.

Rodriguez-Limas,W.A. Flores-Samaniego,B. de la Mora G. Ramirez,O.T. Palomares,L.A. 2009. Genotypification of bovine group A rotavirus in Mexico Vaccine, 27, 6411-6414.

Baez,A. Flores,N. Bolivar,F. Ramirez,O.T. 2009. Metabolic and transcriptional response of recombinant Escherichia coli to elevated dissolved carbon dioxide concentrations Biotechnology and Bioengineering, 104, 102-110.

Palomares,L.A. Ramirez,O.T. 2009. Challenges for the production of virus-like particles in insect cells: the case of rotavirus-like particles Biochemical Engineering Journal, 45, 158-167.

Caspeta,L. Flores,N. Perez,N.O. Bolivar,F. Ramirez,O.T. 2009. The effect of heating rate on Escherichia coli metabolism, physiological stress, transcriptional response, and production of temperature-induced recombinant protein: a scale-down study Biotechnology and Bioengineering, 102, 468-482.

Rodriguez-Galvan,A. Heredia,A. Plascencia-Villa,G. Ramirez,O.T. Palomares,L.A. Basiuk,V.A. 2008. Scanning Tunneling Microscopy of Rotavirus VP6 Protein Self-Assembled into Nanotubes and Nanospheres Journal of Scanning Probe Microscopy, 3, 25-31.

Chavez-Bejar,M.I. Lara,A.R. Lopez,H. Hernandez-Chavez,G. Martinez,A. Ramirez,O.T. Bolivar,F. Gosset,G. 2008. Metabolic engineering of Escherichia coli for L-tyrosine production by the expression of the genes coding for the chorismate mutase domain from native P-protein and a cyclohexadienyl dehydrogenase from Zymomonas mobilis Applied and Environmental Microbiology, 74, 3284-3290.

Martinez,K. de Anda,R. Hernandez,G. Escalante,A. Gosset,G. Ramirez,O.T. Bolivar,F.G. 2008. Coutilization of glucose and glycerol enhances the production of aromatic compounds in an Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system Microbial Cell Factories, 7, 1.

Lara,A.R. Caspeta,L. Gosset,G. Bolivar,F. Ramirez,O.T. 2008. Utility of an Escherichia coli strain engineered in the substrate uptake system for improved culture performance at high glucose and cell concentrations: An alternative to fed-batch cultures Biotechnology and Bioengineering, 99, 893-901.

Flores,N. Leal,L. Sigala,J.C. de Anda,R. Escalante,A. Martinez,A. Ramirez,O.T. Gosset,G. Bolivar,F. 2007. Growth Recovery on Glucose under Aerobic Conditions of an Escherichia coli Strain Carrying a Phosphoenolpyruvate:Carbohydrate Phosphotransferase System Deletion by Inactivating arcA and Overexpressing the Genes Coding for Glucokinase and Galactose Permease Journal of Molecular Microbiology and Biotechnology, 13, 105-116.

Mena,J.A. Ramirez,O.T. Palomares,L.A. 2007. Population kinetics during simultaneous infection of insect cells with two different recombinant baculoviruses for the production of virus-like particles BMC Biotechnology, 7, 39.

Serrato,J.A. Hernandez,V. Estrada-Mondaca,S. Palomares,L.A. Ramirez,O.T. 2007. Differences in the glycosylation profile of a monoclonal antibody produced by hybridomas cultured in serum-supplemented, serum-free or chemically defined media Biotechnology and Applied Biochemistry, 47, 113-124.

Lara,A.R. Galindo,E. Ramirez,O.T. Palomares,L.A. 2006. Living With Heterogeneities in Bioreactors: Understanding the Effects of Environmental Gradients on Cells Molecular Biotechnology, 34, 355-382.

Benavides,J. Mena,J.A. Cisneros-Ruiz,M. Ramirez,O.T. Palomares,L.A. Rito-Palomares,M. 2006. Rotavirus-like particles primary recovery from insect cells in aqueous two-phase systems Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 842, 48-57.

Lara,A.R. Vazquez-Limon,C. Gosset,G. Bolivar,F. Lopez-Mungua,A. Ramirez,O.T. 2006. Engineering Escherichia coli to improve culture performance and reduce formation of by-products during recombinant protein production under transient intermittent anaerobic conditions Biotechnology and Bioengineering, 94, 1164-1175.

de Anda,R. Lara,A.R. Hernandez,V. Hernandez-Montalvo,V. Gosset,G. Bolivar,F. Ramirez,O.T. 2006. Replacement of the glucose phosphotransferase transport system by galactose permease reduces acetate accumulation and improves process performance of Escherichia coli for recombinant protein production without impairment of growth rate Metabolic Engineering, 8, 281-290.

Mena,J.A. Ramirez,O.T. Palomares,L.A. 2006. Intracellular distribution of rotavirus structural proteins and virus-like particles expressed in the insect cell-baculovirus system Journal of Biotechnology, 122, 443-452.

Lara,A.R. Leal,L. Flores,N. Gosset,G. Bolivar,F. Ramirez,O.T. 2006. Transcriptional and metabolic response of recombinant Escherichia coli to spatial dissolved oxygen tension gradients simulated in a scale-down system Biotechnology and Bioengineering, 93, 372-385.

Priego-Jimenez,R. Pena,C. Ramirez,O.T. Galindo,E. 2005. Specific growth rate determines the molecular mass of the alginate produced by Azotobacter vinelandii Biochemical Engineering Journal, 25, 187-193.

Mena,J.A. Ramirez,O.T. Palomares,L.A. 2005. Quantification of rotavirus-like particles by gel permeation chromatography Journal of Chromatography B, 824, 267-276.

Lipscomb,M.L. Palomares,L.A. Hernandez,V. Ramirez,O.T. Kompala,D.S. 2005. Effect of Production Method and Gene Amplification on the Glycosylation Pattern of a Secreted Reporter Protein in CHO Cells Biotechnology Progress, 21, 40-49.

Sandoval-Basurto,E.A. Gosset,G. Bolivar,F. Ramirez,O.T. 2005. Culture of Escherichia coli under dissolved oxygen gradients simulated in a two-compartment scale-down system: Metabolic response and production of recombinant protein Biotechnology and Bioengineering, 89, 453-463.

Estrada-Mondaca,S. Delgado-Bustos,L.A. Ramirez,O.T. 2005. Mannosamine supplementation extends N-acetylglucosaminylation of r-human-SeAP produced in Trichoplusia in insect cell culture Biotechnology and Applied Biochemistry, 42, 25-34.

Cortes,G. Trujillo-Roldan,M.A. Ramirez,O.T. Galindo,E. 2005. Production of -galactosidase by Kluyveromyces marxianus under oscillating dissolved oxygen tension Process Biochemistry, 40, 773-778.

Flores,N. de Anda,R. Flores,S. Escalante,A. Hernandez,G. Martinez,A. Ramirez,O.T. Gosset,G. Bolivar,F. 2004. Role of Pyruvate Oxidase in Escherichia coli Strains Lacking the Phosphoenolpyruvate:Carbohydrate Phosphotransferase System Journal of Molecular Microbiology and Biotechnology, 8, 209-221.

Serrato,J.A. Palomares,L.A. Meneses-Acosta,A. Ramirez,O.T. 2004. Heterogeneous conditions in dissolved oxygen affect N-glycosylation but not productivity of a monoclonal antibody in hybridoma cultures Biotechnology and Bioengineering, 88, 176-188.

Palomares,L.A. Lopez,S. Ramirez,O.T. 2004. Utilization of oxygen uptake rate to assess the role of glucose and glutamine in the metabolism of infected insect cell cultures Biochemical Engineering Journal, 19, 87-93.

De Leon,A. Hernandez,V. Galindo,E. Ramirez,O.T. 2003. Effects of dissolved oxygen tension on the production of recombinant penicillin acylase in Escherichia coli Enzyme and Microbial Technology, 33, 689-697.

Mena,J.A. Ramirez,O.T. Palomares,L.A. 2003. Titration of Non-Occluded Baculovirus Using a Cell Viability Assay Biotechniques, 34, 260-264.

Palomares,L.A. Lopez,S. Ramirez,O.T. 2002. Strategies for manipulating the relative concentration of recombinant rotavirus structural proteins during simultaneous production by insect cells Biotechnology and Bioengineering, 78, 635-644.

Trujillo-Roldan,M.A. Pena,C. Ramirez,O.T. Galindo,E. 2001. Effect of Oscillating Dissolved Oxygen Tension on the Production of Alginate by Azotobacter vinelandii Biotechnology Progress, 17, 1042-1048.

De Leon,A. Barba de la Rosa,A.P. Mayani,H. Galindo,E. Ramirez,O.T. 2001. Two useful dimensionless parameters that combine physiological, operational and bioreactor design parameters for improved control of dissolved oxygen Biotechnology Letters, 23, 1051-1056.

Petricevich,V.L. Palomares,L.A. Gonzalez,M. Ramirez,O.T. 2001. Parameters that determine virus adsorption kinetics: toward the design of better infection strategies for the insect cell Enzyme and Microbial Technology, 29, 52-61.

Meneses-Acosta,A. Mendonca,R. Merchant,H. Covarrubias,L. Ramirez,O. 2001. 3.0.CO;2-3> Comparative characterization of cell death between Sf9 insect cells and hybridoma cultures Biotechnology and Bioengineering, 72, 441-457.

Palomares,L.A. Pedroza,J.C. Ramirez,O.T. 2001. Cell size as a tool to predict the production of recombinant protein by the insect-cell baculovirus expression system Biotechnology Letters, 23, 359-364.

Palomares,L.A. Gonzalez,M. Ramirez,O.T. 2000. Evidence of Pluronic F-68 direct interaction with insect cells: impact on shear protection, recombinant protein, and baculovirus production* Enzyme and Microbial Technology, 26, 324-331.

Ramirez,O.T. Quintero,R. 1999. Pharmaceutical biotechnology emerges in Mexico Nature Biotechnology, 17, 934-934.

Mendonca,R.Z. Palomares,L.A. Ramirez,O.T. 1999. An insight into insect cell metabolism through selective nutrient manipulation Journal of Biotechnology, 72, 61-75.

Mayani,H. Gutierrez-Rodriguez,M. Espinoza,L. Lopez-Chalini,E. Huerta-Zepeda,A. Flores,E. Sanchez-Valle,E. Luna-Bautista,F. Valencia,I. Ramirez,O.T. 1998. Kinetics of hematopoiesis in Dexter-type long-term cultures established from human umbilical cord blood cells Stem Cells, 16, 127-135.

Martinez,A. Ramirez,O.T. Valle,F. 1998. Effect of growth rate on the production of beta-galactosidase from Escherichia coli in Bacillus subtilis using glucose- limited exponentially fedbatch cultures Enzyme and Microbial Technology, 22, 520-526.

De Leon,A. Mayani,H. Ramirez,O.T. 1998. Design, characterization and application of a minibioreactor for the culture of human hematopoietic cells under controlled conditions Cytotechnology, 28, 127-138.

Martinez,A. Ramirez,O.T. Valle,F. 1997. Improvement of culture conditions to overproduce beta-galactosidase from Escherichia coli in Bacillus subtilis Applied Microbiology and Biotechnology, 47, 40-45.

Villarreal,M.L. Arias,C. Vega,J. FeriaVelasco,A. Ramirez,O.T. Nicasio,P. Rojas,G. Quintero,R. 1997. Large-scale cultivation of Solanum chrysotrichum cells: Production of the antifungal saponin SC-1 in 10-l airlift bioreactors Plant Cell Reports, 16, 653-656.

Higareda,A.E. Possani,L.D. Ramirez,O.T. 1997. 3.0.CO;2-H> The use of culture redox potential and oxygen uptake rate for assessing glucose and glutamine depletion in hybridoma cultures Biotechnology and Bioengineering, 56, 555-563.

Villarreal,M.L. Arias,C. FeriaVelasco,A. Ramirez,O.T. Quintero,R. 1997. Cell suspension culture of Solanum chrysotrichum (Schldl.) Plant Cell, Tissue and Organ Culture, 50, 39-44.

Palomares,L.A. Ramirez,O.T. 1996. The effect of dissolved oxygen tension and the utility of oxygen uptake rate in insect cell culture Cytotechnology, 22, 225-237.

Ospina,S. Barzana,E. Ramirez,O.T. Lopez-Munguia,A. 1996. Effect of pH in the synthesis of ampicillin by penicillin acylase Enzyme and Microbial Technology, 19, 462-469.

De Leon,A. Galindo,E. Ramirez,O.T. 1996. A postfermentative stage improves penicillin acylase production by a recombinant E-coli Biotechnology Letters, 18, 927-932.

Ospina,S. Merino,E. Ramirez,O.T. Lopez-Munguia,A. 1995. Recombinant whole cell penicillin acylase biocatalyst: Production, characterization and use in the synthesis and hydrolysis of antibiotics Biotechnology Letters, 17, 615-620.

Ramirez,O.T. Flores,E. Galindo,E. 1995. PRODUCTS AND BIOPROCESSES BASED ON GENETICALLY-MODIFIED ORGANISMS.Asia-Pacific Journal Of Molecular Biology And Biotechnology, 3, 165-197 Correction 4 (1) U5.

Ramirez,O.T. Zamora,R. Quintero,R. Lopez-Munguia,A. 1994. Exponentially fed-batch cultures as an alternative to chemostats: the case of penicillin acylase production by recombinant E. coli Enzyme and Microbial Technology, 16, 895-903.

Higareda,A.E. Possani,L.D. Ramirez,O.T. 1994. Metabolic and kinetic studies of hybridomas in exponentially fed-batch cultures using T-flasks Cytotechnology, 15, 73-86.

Ramirez,O.T. Zamora,R. Espinosa,G. Merino,E. Bolivar,F. Quintero,R. 1994. Kinetic study of penicillin acylase production by recombinant E. Coli in batch cultures Process Biochemistry, 29, 197-206.

Ramirez,O.T. Mutharasan,R. 1992. Effect of serum on the plasma membrane fluidity of hybridomas: an insight into its shear protective mechanism Biotechnology Progress, 8, 40-50 * .

Ramirez,O.T. Sureshkumar,G.K. Mutharasan,R. 1990. Bovine colostrum or milk as a serum substitute for the cultivation of a mouse hybridoma Biotechnology and Bioengineering, 35, 882-889 * .

Ramirez,O.T. Mutharasan,R. 1990. The role of the plasma membrane fluidity on the shear sensitivity of hybridomas grown under hydrodynamic stress Biotechnology and Bioengineering, 36, 911-920 * .

Ramirez,O.T. Mutharasan,R. 1990. Cell cycle- and growth phase-dependent variations in size distribution, antibody productivity, and oxygen demand in hybridoma cultures Biotechnology and Bioengineering, 36, 839-848 * .

Ramirez,O.T. Mutharasan,R. 1989. Physical immobilization characteristics of a hybridoma in a glass bead packed bed reactor Biotechnology and Bioengineering, 33, 1072-1076 * .

Ramirez,O.T. Mutharasan,R. Magee,W.E. 1987. A novel immobilized hybridoma reactor for the production of monoclonal antibodies Biotechnology Techniques, 1, 245-250 * .

Libros y capítulos recientes

Palomares,L.A. Ramirez,O.T. 2019. Hydrodynamic stress and heterogeneities in animal cell culture en: Moo-Young,M. Comprehensive Biotechnology. pags. 108-118

Palomares,L.A. Srivastava,I.K. Ramirez,O.T. Cox,M.M.J. 2018. Glycobiotechnology of the Insect Cell-Baculovirus Expression System Technology en: Reichl,U. Advances in Biochemical Engineering/Biotechnology. Glycobiology. Springer.

Arias-Ortiz,C.F. Arteaga-Vazquez,M.A. Barrera-Saldana,H.A. Galindo-Fentanes,E. Gracia-Gasca,A. Herrera-Estrella,L.R. Higuera-Ciapara,I. Larque-Saavedra,F.A. Lopez-Munguia Canales,A. Ramirez-Reivich,O.T. Revah-Moiseev,S. Soberon-Mainero,X. Torres-Pacheco,I. Uribe de la Mora,J.M. Vazquez-Ramos,J.M. Viniegra-Gonzalez,G. 2017. Transgénicos: Grandes beneficios, ausencia de daños y mitos Comité de Biotecnología, Academia Mexicana de Ciencias-UNAM Instituto de Biotecnologia.

Lara,A. Palomares,L.A. Ramirez,O.T. 2016. Scale-Down: Simulating Large-Scale Cultures in the Laboratory en: Wittmann,C. Industrial Biotechnology Products and Processes. Weinheim, Alemania. Wiley-VCH Verlag GmbH & Co. KGaA. pags. 55-79

Palomares,L.A. Realpe,M. Ramirez,O.T. 2015. An Overview of Cell Culture Engineering for the Insect Cell-Baculovirus Expression Vector System (BEVS) en: Al-Rubeai,M. Animal Cell Culture. Springer International Publishing. pags. 501-519

Lara,A.R. Ramirez,O.T. 2012. Plasmid DNA Production for Therapeutic Applications en: Lorence,A. Recombinant Gene Expression. Springer. pags. 271-303

Bolivar-Zapata,F.G. Arias-Ortiz,C.F. Arriaga-Arellano,E. Barrera-Saldaña,H. de la Torres-Martinez,M. Espinosa-Fernandez,J. Galindo-Fentanes,E. Galvez-Mariscal,A. Gracia-Gasca,A. Herrera-Estrella,L. Larque-Saavedra,A. Lopez-Munguia Canales,A. Noyola-Robles,A. Paredes-Lopez,O. Ramirez-Reivich,O.T. Revah-Moiseev,S. Soberon-Mainero,J. Soberon-Mainero,X. Torres-Pacheco,I. Uribe de la Mora,J. Viniegra-Gonzalez,G. 2011. Por un uso responsable de los organismos genéticamente modificados Mexico:. Academia Mexicana de las Ciencias.

Palomares,L.A. Lara,A. Ramirez,O.T. 2010. Bioreactor Scale-Up en: Flickinger,M. Encyclopedia of Industrial Biotechnology Bioprocess, Bioseparation and Cell Technology. New York. John Wiley and Sons.

Palomares,L.A. Lara,A. Ramirez,O.T. 2010. Bioreactor Scale-Down en: Flickinger,M. Encyclopedia of Industrial Biotechnology. New York. John Wiley and Sons.

Palomares,L.A. Valdez-Cruz,N.A. Ramirez,O.T. 2008. Superando los retos del cultivo de células animales a través de la bioingeniería en: *agustin Una ventana al quehacer científico. Instituto de Biotecnología de la UNAM 25 aniversario, cap 33. Mexico, D.F.. UNAM. pags. 385-398

Palomares,L.A. Ramirez,O.T. 2007. Glicosilación de proteínas recombinantes: Importancia y relación con las condiciones de bioproceso en: Juaresti,E. Diseño y producción de fármacos. Mexico. El Colegio Nacional. pags. 95-123

Palomares,L.A. Estrada-Mondaca,S. Ramirez,O.T. 2006. Principles and applications of the insect cell-baculovirus expression vector system en: Ozturk,S.S. CELL CULTURE TECHNOLOGY FOR PHARMACEUTICAL AND CELL-BASED THERAPIES. Boca Raton, Florida. CRC PRESS. pags. 627-692

Palomares,L.A. Estrada-Mondaca,S. Ramirez,O.T. 2004. Production of recombinant proteins: challenges and solutions en: Lorence,A. Recombinant Gene Expression Reviews and Protocols. pags. 15-52

Ramirez,O.T. 2004. Ingenieria bioquimica en: *bolivar Fundamentos y casos exitosos de la biotecnologia moderna. Mexico. El Colegio Nacional. pags. 249-296

Ramirez,O.T. 2004. Biotecnología farmacéutica moderna en México El caso de Probiomed s.a. de c. V. en: *bolivar Fundamentos y casos exitosos de la biotecnologia moderna. Mexico. El Colegio Nacional. pags. 391-426

Ramirez,O.T. 2003. Nuevos bioprocesos en: *agustin Fronteras de la Biología en los Inicios del Siglo XXI, Módulo VII.. Mexico,D.F.. El Colegio Nacional. pags. 93-121

Gosset,G. Bolivar,F. Soberon,X. Lopez-Munguia,A. Valderrama,B. Ayala,M. Vazquez,R. Ramirez,O.T. Alagon,A. Possani,L.D. 2003. Fronteras de la Biología en los Inicios del Siglo XXI. Módulo VII: Ingeniería Celular, Biodiversidad e Industria. Mexico, D.F.: El Colegio Nacional.

Arias,C.F. Arriaga,E. Barrera,H. Bolivar,F. Bosch,P. De la Torre,M. Espinosa,J. Galindo,E. Galvez,A. Garcia,A. Herrera,L.R. Larque,A. Lopez-Munguia,A. Loyola,A. Ortega,R. Paredes.O. Ramirez,O.T. Revah,S. Serratos,J.A. Soberon,J. Soberon,X. Torres,I. Uribe,J. Viniegra,G. 2003. Recomendaciones para el Desarrollo y Consolidación de la Biotecnología en México Mexico,D.F.: Academia Mexicana de Ciencias, A.C..

Palomares,L.A. Kuri Breña,K. Ramirez,O.T. 2002. Industrial recombinant protein production en: Gobaisi,A. Encyclopedia of Life Support Systems. Oxford. EOLSS Publishers. pags. 1-48

Ortega,R. Arriaga,E. Bosch,P. Espinosa,J. Galvez,A. Ramirez,O.T. 2001. Marco legal e institucional en: *bolivar Biotecnologia moderna para el desarrollo de Mexico en el siglo XXI. Mexico. SEP/CONACYT. pags. 71-116

Ramirez,O.T. 2001. Biotecnologia e industria farmaceutica en: *bolivar Biotecnologia moderna para el desarrollo de Mexico en el siglo XXI. Mexico. SEP/CONACYT. pags. 249-264

Palomares,L.A. Ramirez,O.T. 2000. Bioreactor scale-down en: Spier,R.E. Encyclopedia of cell technology. Wiley. pags. 174-183

Palomares,L.A. Ramirez,O.T. 2000. Bioreactor scale-up en: Spier,R.E. Encyclopedia of Cell Technology. John Wiley and Sons. pags. 183-201

Meneses,A. Gomez,A. Ramirez,O.T. 1999. Feedback control of redox potential in hybridoma cell culture en: Wurm,F. Animal Cell Technology: Products from Cells, Cells As Products. pags. 23-+

Palomares,L.A. Lopez,S. Ramirez,O.T. 1999. Multiplicity and time of infection as tools to maximise virus-like particle production by insect cells en: Wurm,F. Animal cell technology: products form cells, cells as products. Kluwer. pags. 347-349

Villarreal,M.L. Arias,C.F. Feria,A. Ramirez,O.T. Quintero,R. 1998. Production of a new antimycotic agent by plant cell culture en: *tonatiuh Advances in Bioprocess Engineering II. Dordrecht ; London. Kluwer,. pags. 83-95

Galindo,E. Ramirez,O.T. 1998. Advances in Bioprocess Engineering II. Dordrecht, London:. Kluwer.

Ospina,S. Barzana,E. Ramirez,O.T. Lopez-Munguia,A. 1996. C en: mper,J. Progress in Biotechnology. pags. 464-471

Gomez,A. Rodriguez,M. Ospina,S. Zamora,R. Merino,E. Bolivar,F. Ramirez,O.T. Quintero,R. Lopez-Munguia,A. 1994. Strategies in the design of a penicillin acylase process en: *tonatiuh Kluwer Academic. pags. 29-40

Otras Publicaciones

Alcala,A.C. Maravillas,J.L. Meza,D. Ramirez,O.T. Ludert,J.E. Palomares,L.A. 2019. The dengue virus non-structural protein 1 (NS1) use the scavenger receptor B1 as cell receptor in human hepatic and mosquito cells bioRxiv, Preprint posted December 11, 2019, 871988.

Borja,G.M. Ramirez,O.T. Lara,A.R. 2013. Vacunas de ADN Plasmídico: Una Herramienta Terapéutica Emergente Biotecnologia, 17, 87-109.

Plascencia-Villa,G. Palomares,L.A. Ramirez,O.T. 2011. Síntesis y Ensamblaje de Nanomateriales Usando Proteínas Virales Como Templados Biotecnologia, 15, 50-77.

Chavez-Pena,C. Ayala-Mendivil,N.A. Ramirez,O.T. Palomares,L.A. 2010. Efecto de la densidad celular y la multiplicidad de infeccion sobre la produccion de baculovirus recombinantes en cultivos de celulas de insecto TIP revista especializada en Ciencias Quimico-Biologicas, 13, 65-72.

Plascencia-Villa,G. Ramirez-Reivich,O.T. Palomares-Aguilera,L. 2009. Nanobiomateriales creados a partir de virus Hypatia, 31, .

Valdez-Cruz,N.A. Ramirez-Reivich,O.T. Trujillo-Roldan,M.A. 2009. Proteínas recombinantes terapéuticas, una esperanza contra enfermedades crónicas. Hypatia, 32, .

Rodriguez-Limas,W.A. Ramirez,O.T. Palomares,L.A. 2007. Identificación de genotipos de rotavirus bovino prevalecientes en México como herramienta para el desarrollo de una vacuna recombinante Biotecnologia, 10, 34-41.

Plasencia,F.J. Ramirez,O.T. Estrada,S. 2005. Identificacion y clonacion del ARNm de la 2,6.sialiltransferasa de Anopheles albimanus.Entomología Mexicana, 4, 791-795.

Ramirez,O.T. Piret,J. Krummen,L. Konstantinov,K. 2005. Cell Culture Engineering IX Biotechnology Progress, 21, 1-1.

Palomares,L.A. Ramirez,O.T. 2002. El sistema de celulas de insecto-baculovirus: una alternativa para la produccion de pseudo-particulas virales y otras proteinas recombinantes.Biotecnologia, 6, 16-29.

Palomares,L.A. Ramirez,O.T. 2002. Complex N-glycosylation of Recombinant Proteins by Insect Cells Bioprocessing, 1, 70-73.

de Luna,A. Levy,K. Ramirez,O.T. 2000. El congreso de Huatulco 99: una sinfonia ejecutada por todos los miembros de la SMBB.Biotecnologia, 5, 4-10.

Galindo,E. de la Torre,M. Ramirez,O. Gutierrez,R. Garcia-Garibay,M. Torres,L. 2000. Informe final y mensaje de la Mesa Directiva de la Sociedad Mexicana de Biotecnologia y Bioingenieria Biotecnologia Aplicada, 17, 202-204.

Galindo,E. Ramirez,O.T. 1998. Bioprocess engineering Trends in Biotechnology, 16, 282-283.

Patentes

Ponce Laura Grecia , Martínez Mejía Luz María , Ramírez Reivich Octavio Tonatiuh , Martínez Jiménez Alfredo , Bolívar Zapata Francisco Gonzalo , Gosset Lagarda Guillermo . 2013 Cepas de Escherichia coli con alta capacidad para la producción de plásmidos y su uso en la producción de vacunas de ADN.UNAM México. (en trámite)

Laura Alicia Palomares Aguilera, Ricardo Martín Castro Acosta, Octavio Tonatiuh Ramírez Reivich, Alma Luisa Revilla Vázquez 2009 Método analítico para la cuantificación diferenciada de estructuras virales proteicas multiméricas.UNAM México.

O. T. Ramírez Reivich, G. Plascencia Villa, L. A. Palomares A., Y. A. Mena Mendez y J. M. Saniger Blesa 2008 Uso de proteínas multiméricas virales como templados para la construcción de nanobiomateriales.UNAM PCT. (en trámite)

E. Galindo , O. T. Ramírez y A. de León 2007 Proceso en dos etapas para la producción de células conteniendo proteína madurada con actividad biológica.UNAM México.

A. R. Lara R., M. de C. Vázquez L., G. Gosset L., F. G. Bolívar Z., A. Lopez-Munguia C. y O. T. Ramírez R 2006 Estrategia para generar células insensibles a condiciones heterogéneas en biorreactores industriales a través de mutaciones de vías metabólicas anaerobias.UNAM México. (en trámite)

* Indica publicación con otra institución de adscripción